Detailed information of TA system TA211285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1097679..1099368
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A3A6SPN4
• Locus tag: KZW90_RS05320
• Protein ID: WP_063085362.1
• Coordinates: 1098448..1099368 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: KZW90_RS05315
• Protein ID: WP_063502601.1
• Coordinates: 1097679..1098461 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS05310 (1094297)	1094297..1097395	-	3099	WP_063085364.1	type I restriction endonuclease	-
KZW90_RS05315 (1097679)	1097679..1098461	+	783	WP_063502601.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
KZW90_RS05320 (1098448)	1098448..1099368	+	921	WP_063085362.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KZW90_RS05325 (1099802)	1099802..1100686	-	885	WP_001119037.1	LysR family transcriptional regulator	-
KZW90_RS05330 (1100951)	1100951..1102009	+	1059	WP_122987789.1	alpha/beta hydrolase	-
KZW90_RS05335 (1102151)	1102151..1103278	+	1128	WP_042028035.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34587.06 Da
• Isoelectric Point: 7.3238

>T211285 WP_063085362.1 NZ_CP080233:1098448-1099368 [Escherichia coli O139:H1]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDIERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T211285 NZ_CP104835:1179150-1179527 [Achromobacter xylosoxidans]
ATGATCCTGGTTGACACTTCCATCTGGATAGACCATTTATCCGACGGCGAGCCATGTCTGGTGCGCTTGCTGGAGGAAGA
AAGTGTATTGATGCACCCCTTCATCGCGGGTGAATTGGCGCTGGGTTCGTTGGGCCGGCGTGATGTCGTATTGGGTGCGA
TGTCGCTATTGCCTCAAGTCGTGCGTGCGAGTCATGACGAAGTCATGCACTTTCTTCATAGCGAGAGATTGTTTGGAAAG
GGCATTGGTTATGTGGATTTACACTTGTTGGCGTCCACGCGACTGACACCTGGCGCGGCGCTATGGACCAGGGACAAACG
GCTGCAGGCACTCGCCACCACCTTGAGCCTGGCGGTTGATCCCCCGAGATATCATTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29094.32 Da
• Isoelectric Point: 8.1223

>AT211285 WP_063502601.1 NZ_CP080233:1097679-1098461 [Escherichia coli O139:H1]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGRPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDCQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT211285 NZ_CP104835:1178954-1179163 [Achromobacter xylosoxidans]
GTGCGTACGACTGTGACGCTCGATGACGACCTGCTCGAATTGGCCCGTACCTATACGGGCGTCCAGGAGACATCGGCGCT
CGTAACGCGAGCGTTGACCCAGCTCGTGCAGAAAGAGGCCGCGAAGCGGTTGGCCAAGCTTGGTGGTAGTTCGCCCAAGT
TGAAGCCTATCCCGCGGCGGCAGACGGAAACCTGCAATGATCCTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A3A6SPN4