Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 722928..723504 Replicon chromosome
Accession NZ_CP080233
Organism Escherichia coli O139:H1 strain P15-25

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KZW90_RS03455 Protein ID WP_001297643.1
Coordinates 722928..723215 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KZW90_RS03460 Protein ID WP_000063160.1
Coordinates 723202..723504 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW90_RS03435 (719181) 719181..720461 - 1281 WP_001037400.1 DUF445 domain-containing protein -
KZW90_RS03440 (720502) 720502..721734 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
KZW90_RS03445 (721880) 721880..721975 - 96 Protein_663 dienelactone hydrolase family protein -
KZW90_RS03450 (722032) 722032..722729 + 698 Protein_664 IS1 family transposase -
KZW90_RS03455 (722928) 722928..723215 + 288 WP_001297643.1 BrnT family toxin Toxin
KZW90_RS03460 (723202) 723202..723504 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KZW90_RS03465 (723538) 723538..724494 - 957 WP_001297640.1 GTPase -
KZW90_RS03470 (724505) 724505..724708 - 204 WP_000467859.1 YbdD/YjiX family protein -
KZW90_RS03475 (724758) 724758..726908 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T211283 WP_001297643.1 NZ_CP080233:722928-723215 [Escherichia coli O139:H1]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T211283 NZ_CP104798:179337-179753 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT211283 WP_000063160.1 NZ_CP080233:723202-723504 [Escherichia coli O139:H1]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT211283 NZ_CP104798:179110-179340 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References