Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 49499..50100 Replicon plasmid pP136-6
Accession NZ_CP080229
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KZW89_RS28165 Protein ID WP_001216045.1
Coordinates 49720..50100 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KZW89_RS28160 Protein ID WP_001190712.1
Coordinates 49499..49720 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS28150 (KZW89_28150) 46582..47763 - 1182 WP_120150456.1 restriction endonuclease subunit S -
KZW89_RS28155 (KZW89_28155) 47760..49316 - 1557 WP_086186057.1 type I restriction-modification system subunit M -
KZW89_RS28160 (KZW89_28160) 49499..49720 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZW89_RS28165 (KZW89_28165) 49720..50100 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZW89_RS28170 (KZW89_28170) 50105..50284 + 180 WP_000113018.1 hypothetical protein -
KZW89_RS28175 (KZW89_28175) 50312..51355 + 1044 WP_072660937.1 DUF968 domain-containing protein -
KZW89_RS28180 (KZW89_28180) 51444..51896 + 453 WP_032192895.1 Late promoter-activating protein -
KZW89_RS28185 (KZW89_28185) 51982..53175 + 1194 WP_000219618.1 hypothetical protein -
KZW89_RS28190 (KZW89_28190) 53175..54659 + 1485 WP_000124159.1 hypothetical protein -
KZW89_RS28195 (KZW89_28195) 54881..55000 + 120 WP_071527722.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74646 74646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T211278 WP_001216045.1 NZ_CP080229:49720-50100 [Escherichia coli O141:H4]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T211278 NZ_CP104796:c4698052-4697768 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT211278 WP_001190712.1 NZ_CP080229:49499-49720 [Escherichia coli O141:H4]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT211278 NZ_CP104796:c4698284-4698042 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References