Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 14767..15314 Replicon plasmid pP136-3
Accession NZ_CP080226
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag KZW89_RS26430 Protein ID WP_001384452.1
Coordinates 15036..15314 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag KZW89_RS26425 Protein ID WP_000079941.1
Coordinates 14767..15036 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS26395 (9941) 9941..10399 - 459 Protein_13 transposase -
KZW89_RS26400 (10477) 10477..10626 - 150 Protein_14 transposase -
KZW89_RS26405 (11134) 11134..11352 + 219 WP_001353651.1 heat-stable enterotoxin ST-I group a -
KZW89_RS26415 (12936) 12936..13328 - 393 Protein_17 IS21-like element IS21 family helper ATPase IstB -
KZW89_RS26420 (13328) 13328..14500 - 1173 WP_015056461.1 IS21-like element IS21 family transposase -
KZW89_RS26425 (14767) 14767..15036 + 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
KZW89_RS26430 (15036) 15036..15314 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
KZW89_RS26435 (15623) 15623..16765 + 1143 WP_158649904.1 YiiX/YebB-like N1pC/P60 family cysteine hydrolase -
KZW89_RS28990 (16738) 16738..16842 + 105 Protein_22 hypothetical protein -
KZW89_RS26440 (16854) 16854..17159 + 306 WP_000482654.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa 1..82610 82610
- inside IScluster/Tn - estIa 2268..25722 23454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T211272 WP_001384452.1 NZ_CP080226:15036-15314 [Escherichia coli O141:H4]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T211272 NZ_CP104796:355907-356254 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT211272 WP_000079941.1 NZ_CP080226:14767-15036 [Escherichia coli O141:H4]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT211272 NZ_CP104796:356254-356547 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References