Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 85447..87070 | Replicon | plasmid pP136-1 |
| Accession | NZ_CP080224 | ||
| Organism | Escherichia coli O141:H4 strain P13-6 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | H9TH67 |
| Locus tag | KZW89_RS25020 | Protein ID | WP_000088046.1 |
| Coordinates | 85745..87070 (+) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A5Z6V2D1 |
| Locus tag | KZW89_RS25015 | Protein ID | WP_024136327.1 |
| Coordinates | 85447..85740 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZW89_RS24990 (KZW89_24990) | 81319..82560 | + | 1242 | WP_001053338.1 | VWA domain-containing protein | - |
| KZW89_RS28940 | 82651..82782 | + | 132 | WP_000374059.1 | hypothetical protein | - |
| KZW89_RS24995 (KZW89_24995) | 83123..83404 | - | 282 | WP_000077926.1 | hypothetical protein | - |
| KZW89_RS25000 (KZW89_25000) | 83454..83645 | - | 192 | WP_000398480.1 | hypothetical protein | - |
| KZW89_RS25005 (KZW89_25005) | 83737..84078 | - | 342 | WP_001151575.1 | hypothetical protein | - |
| KZW89_RS25010 (KZW89_25010) | 84451..84843 | + | 393 | WP_000341066.1 | hypothetical protein | - |
| KZW89_RS25015 (KZW89_25015) | 85447..85740 | + | 294 | WP_024136327.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| KZW89_RS25020 (KZW89_25020) | 85745..87070 | + | 1326 | WP_000088046.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| KZW89_RS25025 (KZW89_25025) | 87131..87370 | + | 240 | WP_112005877.1 | hypothetical protein | - |
| KZW89_RS25030 (KZW89_25030) | 87339..90017 | - | 2679 | Protein_84 | Tn3-like element TnAs3 family transposase | - |
| KZW89_RS25035 (KZW89_25035) | 90060..90764 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| KZW89_RS25040 (KZW89_25040) | 90936..91844 | + | 909 | WP_000031017.1 | Mph(B) family macrolide 2'-phosphotransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mph(B) / tet(B) | - | 1..226437 | 226437 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50249.20 Da Isoelectric Point: 6.1927
>T211265 WP_000088046.1 NZ_CP080224:85745-87070 [Escherichia coli O141:H4]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T211265 NZ_CP104790:c59246-58848 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGTCGGCCTGAGAATTGAAGACTGGAGCTGA
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGTCGGCCTGAGAATTGAAGACTGGAGCTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10946.35 Da Isoelectric Point: 10.0925
>AT211265 WP_024136327.1 NZ_CP080224:85447-85740 [Escherichia coli O141:H4]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
Download Length: 294 bp
>AT211265 NZ_CP104790:c59473-59246 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3T0VBU1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z6V2D1 |