Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4488894..4489391 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZW89_RS22430 Protein ID WP_001173343.1
Coordinates 4488894..4489067 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZW89_RS22435 Protein ID WP_000398619.1
Coordinates 4489095..4489391 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS22420 (4485564) 4485564..4487021 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KZW89_RS22425 (4487258) 4487258..4488775 + 1518 WP_001295074.1 lysine--tRNA ligase -
KZW89_RS22430 (4488894) 4488894..4489067 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZW89_RS22435 (4489095) 4489095..4489391 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZW89_RS22440 (4489618) 4489618..4489890 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KZW89_RS22445 (4489902) 4489902..4490132 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KZW89_RS22450 (4490313) 4490313..4491944 + 1632 WP_001216477.1 sensor histidine kinase -
KZW89_RS22455 (4491941) 4491941..4492660 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4456437..4489391 32954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T211264 WP_001173343.1 NZ_CP080223:c4489067-4488894 [Escherichia coli O141:H4]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T211264 NZ_CP104790:55625-55912 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAAGAATGGATGAAACTGGATAAATCCATTCAGCAACAGTTCGC
AAAAAAATTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGAACTAAAAAATTGCTACAAAATCA
AACTTCGTGCATCGGGTTTTCGTCTGGTCTATCAGGTTATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAATAACGTTTATAATCTTGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT211264 WP_000398619.1 NZ_CP080223:c4489391-4489095 [Escherichia coli O141:H4]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT211264 NZ_CP104790:55377-55628 [Escherichia coli]
ATGCCTAATATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAATTGAAAAAAAACCCAATGGCAACAGTCGGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGAAACCAGCCGGCATTCTACTGTGTGCCTGCGGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGATCAGGAGCTGGTGAAACTGGTTACTGAACGTAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References