Detailed information of TA system TA211263

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4477111..4477946
• Replicon: chromosome
• Accession: NZ_CP080223
• Organism: Escherichia coli O141:H4 strain P13-6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KZW89_RS22385
• Protein ID: WP_000854821.1
• Coordinates: 4477569..4477946 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0D0LUA6
• Locus tag: KZW89_RS22380
• Protein ID: WP_001285613.1
• Coordinates: 4477111..4477479 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW89_RS22355 (4474516)	4474516..4474749	+	234	WP_001117568.1	DUF905 family protein	-
KZW89_RS22360 (4474839)	4474839..4475657	+	819	WP_042634191.1	DUF932 domain-containing protein	-
KZW89_RS22365 (4475749)	4475749..4476231	+	483	WP_000206657.1	antirestriction protein	-
KZW89_RS22370 (4476247)	4476247..4476723	+	477	WP_001384108.1	RadC family protein	-
KZW89_RS22375 (4476810)	4476810..4477031	+	222	WP_000692350.1	DUF987 domain-containing protein	-
KZW89_RS22380 (4477111)	4477111..4477479	+	369	WP_001285613.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZW89_RS22385 (4477569)	4477569..4477946	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KZW89_RS22390 (4477943)	4477943..4478140	+	198	Protein_4397	DUF5983 family protein	-
KZW89_RS22395 (4478168)	4478168..4478365	+	198	WP_085949090.1	DUF957 domain-containing protein	-
KZW89_RS22400 (4478450)	4478450..4479292	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
KZW89_RS22405 (4480041)	4480041..4481579	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4456437..4489391	32954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T211263 WP_000854821.1 NZ_CP080223:4477569-4477946 [Escherichia coli O141:H4]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T211263 NZ_CP104790:2049-2534 [Escherichia coli]
ATGGGATGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTCTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTCGGTTTTTACTCACTGGCTACTGGCAGTGTCAACCATACAGAAGCTACAGGCAATCTTCGGCGCAATATG
CCTGACCCCATCCCTGTCATTATACTTGCACGACTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCGGATTT
ACTACATGATGCAGTTCGTCGTTGTTACAGAGTGGCCGAAAATATCGGTGTACGTGCAATTATGGTTCATGCACTTACGG
AAAGCGCAAAACAGTTTTATATCCATCATGGCTTTACACCATCAAAAACTCAGCTGCAGACATTGTTTCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13636.42 Da
• Isoelectric Point: 6.0618

>AT211263 WP_001285613.1 NZ_CP080223:4477111-4477479 [Escherichia coli O141:H4]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQTFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT211263 NZ_CP104790:1777-2061 [Escherichia coli]
ATGATATACGGAGGCTTTATGAAATCAGATGTTCAGCTCAACCTCAGAGCTAGAGAATCACAGCGGATACTCATTGATAC
CGCCGCAGAAATTCTTCATAAGTCACGTACAGACTTCATTCTTGAAATGGCCTGTAAAGCTGCGGAAGATGTTATTCTTG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCAGATGAGCCT
GCCATCGAAAAATTACTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54

Antitoxin

• AlphaFold DB: A0A0D0LUA6