Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2525731..2526369 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KZW89_RS12515 Protein ID WP_000813794.1
Coordinates 2526193..2526369 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KZW89_RS12510 Protein ID WP_001270286.1
Coordinates 2525731..2526147 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS12490 (2520883) 2520883..2521824 - 942 WP_001251304.1 ABC transporter permease -
KZW89_RS12495 (2521825) 2521825..2522838 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KZW89_RS12500 (2522856) 2522856..2524001 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KZW89_RS12505 (2524246) 2524246..2525652 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KZW89_RS12510 (2525731) 2525731..2526147 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KZW89_RS12515 (2526193) 2526193..2526369 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KZW89_RS12520 (2526591) 2526591..2526821 + 231 WP_000494244.1 YncJ family protein -
KZW89_RS12525 (2526913) 2526913..2528874 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KZW89_RS12530 (2528947) 2528947..2529483 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KZW89_RS12535 (2529575) 2529575..2530750 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T211258 WP_000813794.1 NZ_CP080223:c2526369-2526193 [Escherichia coli O141:H4]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T211258 NZ_CP104788:c3918109-3918002 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT211258 WP_001270286.1 NZ_CP080223:c2526147-2525731 [Escherichia coli O141:H4]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT211258 NZ_CP104788:3918157-3918223 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References