Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 842577..843231 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KZW89_RS04085 Protein ID WP_000244777.1
Coordinates 842824..843231 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZW89_RS04080 Protein ID WP_000354046.1
Coordinates 842577..842843 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS04055 (837746) 837746..838489 + 744 WP_000951964.1 SDR family oxidoreductase -
KZW89_RS04060 (838546) 838546..839979 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KZW89_RS04065 (840024) 840024..840335 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KZW89_RS04070 (840499) 840499..841158 + 660 WP_000250274.1 hemolysin III family protein -
KZW89_RS04075 (841354) 841354..842334 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZW89_RS04080 (842577) 842577..842843 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZW89_RS04085 (842824) 842824..843231 + 408 WP_000244777.1 protein YgfX Toxin
KZW89_RS04090 (843271) 843271..843792 - 522 WP_001055874.1 flavodoxin FldB -
KZW89_RS04095 (843904) 843904..844800 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZW89_RS04100 (844825) 844825..845535 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZW89_RS04105 (845541) 845541..847274 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T211246 WP_000244777.1 NZ_CP080223:842824-843231 [Escherichia coli O141:H4]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211246 NZ_CP104788:c1416203-1416096 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211246 WP_000354046.1 NZ_CP080223:842577-842843 [Escherichia coli O141:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211246 NZ_CP104788:1416250-1416316 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References