Detailed information of TA system TA211236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2177376..2177905
• Replicon: chromosome
• Accession: NZ_CP080222
• Organism: Staphylococcus aureus strain NT_8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KZW87_RS10990
• Protein ID: WP_000621175.1
• Coordinates: 2177376..2177738 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KZW87_RS10995
• Protein ID: WP_000948331.1
• Coordinates: 2177735..2177905 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW87_RS10970 (KZW87_10935)	2174354..2175124	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KZW87_RS10975 (KZW87_10940)	2175099..2175578	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KZW87_RS10980 (KZW87_10945)	2175580..2175906	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KZW87_RS10985 (KZW87_10950)	2176025..2177026	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KZW87_RS10990 (KZW87_10955)	2177376..2177738	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KZW87_RS10995 (KZW87_10960)	2177735..2177905	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KZW87_RS11000 (KZW87_10965)	2177990..2179138	-	1149	WP_001281154.1	alanine racemase	-
KZW87_RS11005 (KZW87_10970)	2179204..2179563	-	360	WP_000581197.1	holo-ACP synthase	-
KZW87_RS11010 (KZW87_10975)	2179567..2180058	-	492	WP_072353916.1	PH domain-containing protein	-
KZW87_RS11015 (KZW87_10980)	2180045..2181628	-	1584	WP_001294626.1	PH domain-containing protein	-
KZW87_RS11020 (KZW87_10985)	2181621..2182100	-	480	WP_031590204.1	hypothetical protein	-
KZW87_RS11025 (KZW87_10990)	2182309..2182869	-	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T211236 WP_000621175.1 NZ_CP080222:c2177738-2177376 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T211236 NZ_CP104767:53928-54410 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT211236 WP_000948331.1 NZ_CP080222:c2177905-2177735 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT211236 NZ_CP104767:53674-53940 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1