Detailed information of TA system TA211235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2018853..2019629
• Replicon: chromosome
• Accession: NZ_CP080222
• Organism: Staphylococcus aureus strain NT_8

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KZW87_RS10070
• Protein ID: WP_000031108.1
• Coordinates: 2018853..2019005 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: KZW87_RS10075
• Protein ID: WP_001251230.1
• Coordinates: 2019030..2019629 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW87_RS10055 (KZW87_10020)	2014768..2015589	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
KZW87_RS10060 (KZW87_10025)	2016041..2017426	-	1386	WP_000116229.1	class II fumarate hydratase	-
KZW87_RS10065 (KZW87_10030)	2017622..2018017	-	396	WP_000901023.1	hypothetical protein	-
KZW87_RS10070 (KZW87_10035)	2018853..2019005	-	153	WP_000031108.1	SAS053 family protein	Toxin
KZW87_RS10075 (KZW87_10040)	2019030..2019629	-	600	WP_001251230.1	hypothetical protein	Antitoxin
KZW87_RS10080 (KZW87_10045)	2019788..2020258	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KZW87_RS10085 (KZW87_10050)	2020263..2021390	-	1128	WP_031590038.1	tRNA epoxyqueuosine(34) reductase QueG	-
KZW87_RS10090 (KZW87_10055)	2021541..2022263	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KZW87_RS10095 (KZW87_10060)	2022256..2023713	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T211235 WP_000031108.1 NZ_CP080222:c2019005-2018853 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T211235 NZ_CP104766:c5323543-5323160 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22369.51 Da
• Isoelectric Point: 4.9728

>AT211235 WP_001251230.1 NZ_CP080222:c2019629-2019030 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKYAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT211235 NZ_CP104766:c5323785-5323543 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: X5E2E6