Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1678449..1679047 Replicon chromosome
Accession NC_006510
Organism Geobacillus kaustophilus HTA426

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag GK_RS08640 Protein ID WP_011231143.1
Coordinates 1678718..1679047 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GK_RS08635 Protein ID WP_011231142.1
Coordinates 1678449..1678718 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK_RS08600 1674149..1675129 + 981 WP_011231139.1 ABC transporter permease -
GK_RS08605 1675198..1675665 - 468 WP_021321939.1 hypothetical protein -
GK_RS08610 1675679..1676443 - 765 WP_011231140.1 hypothetical protein -
GK_RS08615 1676570..1677625 + 1056 WP_011231141.1 transposase -
GK_RS08635 1678449..1678718 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GK_RS08640 1678718..1679047 + 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GK_RS08645 1679500..1680333 + 834 WP_011231146.1 pirin family protein -
GK_RS08650 1680429..1681178 + 750 WP_011231147.1 oxygen-insensitive NADPH nitroreductase -
GK_RS08655 1681368..1682111 + 744 WP_011231148.1 NAD-dependent protein deacylase -
GK_RS08660 1682291..1683469 + 1179 WP_011231149.1 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T21123 WP_011231143.1 NC_006510:1678718-1679047 [Geobacillus kaustophilus HTA426]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T21123 NC_006510:1678718-1679047 [Geobacillus kaustophilus HTA426]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT21123 WP_011231142.1 NC_006510:1678449-1678718 [Geobacillus kaustophilus HTA426]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT21123 NC_006510:1678449-1678718 [Geobacillus kaustophilus HTA426]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References