Detailed information of TA system TA211228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 882108..882585
• Replicon: chromosome
• Accession: NZ_CP080222
• Organism: Staphylococcus aureus strain NT_8

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KZW87_RS04285
• Protein ID: WP_000052076.1
• Coordinates: 882108..882296 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KZW87_RS04290
• Protein ID: WP_001059690.1
• Coordinates: 882325..882585 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW87_RS04255 (KZW87_04240)	878143..878619	+	477	WP_000824477.1	hypothetical protein	-
KZW87_RS04260 (KZW87_04245)	878960..879646	+	687	WP_001641381.1	thermonuclease family protein	-
KZW87_RS04265 (KZW87_04250)	880003..880203	+	201	WP_001059082.1	cold-shock protein	-
KZW87_RS04270 (KZW87_04255)	880699..880917	-	219	WP_000005887.1	hypothetical protein	-
KZW87_RS04275 (KZW87_04260)	880979..881263	-	285	WP_031590186.1	hypothetical protein	-
KZW87_RS04280 (KZW87_04265)	881351..881920	-	570	WP_001106652.1	lipoprotein N-acylation protein LnsA	-
KZW87_RS04285 (KZW87_04270)	882108..882296	-	189	WP_000052076.1	hypothetical protein	Toxin
KZW87_RS04290 (KZW87_04275)	882325..882585	-	261	WP_001059690.1	hypothetical protein	Antitoxin
KZW87_RS04295 (KZW87_04280)	882926..883129	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KZW87_RS04300 (KZW87_04285)	883126..883362	-	237	WP_000368687.1	hypothetical protein	-
KZW87_RS04305 (KZW87_04290)	883587..884168	+	582	WP_001140697.1	histidine phosphatase family protein	-
KZW87_RS04310 (KZW87_04295)	884241..884858	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
KZW87_RS04315 (KZW87_04300)	885014..885508	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
KZW87_RS04320 (KZW87_04305)	885907..886329	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
KZW87_RS04325 (KZW87_04310)	886477..887193	+	717	WP_031589686.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T211228 WP_000052076.1 NZ_CP080222:c882296-882108 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T211228 NZ_CP104766:2508170-2508487 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT211228 WP_001059690.1 NZ_CP080222:c882585-882325 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT211228 NZ_CP104766:2508563-2508859 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56