Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 15452..16095 Replicon plasmid pPK8566-117kb
Accession NZ_CP080174
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KZY09_RS24535 Protein ID WP_001044768.1
Coordinates 15452..15868 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KZY09_RS24540 Protein ID WP_001261287.1
Coordinates 15865..16095 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS24520 (11951) 11951..12541 - 591 WP_000194575.1 hypothetical protein -
KZY09_RS24525 (12541) 12541..12798 - 258 WP_000343085.1 hypothetical protein -
KZY09_RS24530 (13152) 13152..15290 + 2139 WP_000350638.1 AAA family ATPase -
KZY09_RS24535 (15452) 15452..15868 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY09_RS24540 (15865) 15865..16095 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY09_RS24545 (16280) 16280..16722 - 443 Protein_19 transposase -
KZY09_RS26065 (16782) 16782..17023 + 242 Protein_20 hypothetical protein -
KZY09_RS24550 (17306) 17306..18190 - 885 WP_000942222.1 helix-turn-helix domain-containing protein -
KZY09_RS24555 (18631) 18631..19305 + 675 WP_232379284.1 helix-turn-helix domain-containing protein -
KZY09_RS24560 (19350) 19350..20099 - 750 WP_106141716.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..117749 117749
- flank IS/Tn - - 16396..16722 326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T211219 WP_001044768.1 NZ_CP080174:c15868-15452 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T211219 NZ_CP104762:c784861-784676 [Enterococcus raffinosus]
ATGGATGCTAAGGAGCTAGCCAAGATTCTTAAAAACGATGGTTGGTATTTTGATTCTCAACGTGGAAGTCATCGCTACTA
CAAACATCCCATAAAAAAGGGCAAAGTACCTGTCCCATTCCACAGTCACAAAGACATCCCTATTGGAACACTCAACAACA
TACTTAAACAAGCTGGCTTAAAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT211219 WP_001261287.1 NZ_CP080174:c16095-15865 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT211219 NZ_CP104762:c784623-784237 [Enterococcus raffinosus]
ATGGTCATTTATTATGCTATGTTCGATTTTGCAGATAATGGGATCAATGTTGTTTTTCCAGATTTGAACAATGCGGCTAC
ATTTGGTGAAAATATGCATGAGGCCCTCTATATGGCTAAAGATTTACTAGCAGGTTGGCTGATTGATGCAGAGGATGAAA
AGCAAGCTTTTCCTTCACCTACTGATCACCGAAGTCTGAGTGTAGCAGCTGGAAATTTATTGATCCCAATTGAAGTTGAT
TTATCCTTTTACCGTAAAAAATTTGAAAGTAAGCCTATCAAAAAAACATTAACTATTCCAAAATATTTAAATGATTTAGG
CAATGAAGCAGGAATCAACTTCTCAGCTACACTAACTGAAGCATTGAAAGAAAAATTAGAAGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References