Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1985..2532 Replicon plasmid pPK8566-117kb
Accession NZ_CP080174
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag KZY09_RS24460 Protein ID WP_001384452.1
Coordinates 2254..2532 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag KZY09_RS24455 Protein ID WP_000079928.1
Coordinates 1985..2254 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS24450 (46) 46..1077 + 1032 WP_000907875.1 plasmid replication initiator RepA -
KZY09_RS24455 (1985) 1985..2254 + 270 WP_000079928.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
KZY09_RS24460 (2254) 2254..2532 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
KZY09_RS24465 (2617) 2617..2964 + 348 WP_219795695.1 DUF6404 family protein -
KZY09_RS24470 (2982) 2982..3572 - 591 WP_000194550.1 hypothetical protein -
KZY09_RS24475 (3572) 3572..3826 - 255 WP_000343103.1 hypothetical protein -
KZY09_RS24480 (4178) 4178..5179 + 1002 WP_000774874.1 DUF4238 domain-containing protein -
KZY09_RS24485 (5355) 5355..5699 - 345 WP_000793307.1 hypothetical protein -
KZY09_RS24490 (6285) 6285..6989 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KZY09_RS24495 (7042) 7042..7362 - 321 Protein_9 NAD(P)H-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..117749 117749
- flank IS/Tn - - 6285..6989 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T211218 WP_001384452.1 NZ_CP080174:2254-2532 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T211218 NZ_CP104762:c340851-340663 [Enterococcus raffinosus]
ATGCCTATGACACAAAGAGAAATGGTTAAGCTCCTTAAGTCTCACGGTTTCAAAAAAGTTGGAGATGAAGGCAAAGGTTC
TCATGTCAAAATGACAAAACCGGGACAGGCGAGACCTATCATTATTCCTCACGGTGAACTAAATAAATATACCGAACGAG
GAATAAAAAAGGATGCCGGCTTACTTTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT211218 WP_000079928.1 NZ_CP080174:1985-2254 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT211218 NZ_CP104762:c340629-340177 [Enterococcus raffinosus]
ATGCTAGTTACCTATCCAGCGCTGTTTTATTATGATGATACAGATAAAACATCAGTACCCTACTCTGTTTTTTTTCCTGA
TATTCATTATGGTGCTACACAAGGTGAAAATATTTCAGATGCGATGTCCATGGCCTCTGAATTTCTCGGAATTGCAGTGG
CTTCAATGGTTGAAGATAACGAAAAAGTTCCTACCCCTTCAAATATTCATAATCTGTCACTTGTTGATAATAACCCCTTT
ATAGACGATAAAGATTTCGACTTACAATTCGACCCTGAAAAATCCTTTATCTCGATGGTTACAGTTGATATTTCTCAATA
CCTTGGTGAGCAAGAACCAGTAAAAAAAACACTAACAATACCTAAATGGGCTGACCGCTTAGGTAAAGAGTTGCATCTTA
ATTTTTCAAAAACATTAACAGATGCAATCGCAAATAAACATATTGAAGTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References