Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 147218..147861 Replicon plasmid pPK8566-169kb
Accession NZ_CP080173
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KZY09_RS24350 Protein ID WP_001034044.1
Coordinates 147445..147861 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KZY09_RS24345 Protein ID WP_001261286.1
Coordinates 147218..147448 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS24330 (142355) 142355..142585 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KZY09_RS24335 (142582) 142582..142998 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KZY09_RS24340 (143043) 143043..146837 - 3795 WP_001144731.1 hypothetical protein -
KZY09_RS24345 (147218) 147218..147448 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY09_RS24350 (147445) 147445..147861 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY09_RS24355 (147936) 147936..149501 + 1566 WP_001128474.1 AAA family ATPase -
KZY09_RS24360 (149486) 149486..150508 + 1023 WP_000361402.1 helicase UvrD -
KZY09_RS24365 (150762) 150762..151448 - 687 Protein_162 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / tet(A) / sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..169561 169561
- flank IS/Tn - - 150762..151109 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T211217 WP_001034044.1 NZ_CP080173:147445-147861 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T211217 NZ_CP104761:5982-6398 [Kalamiella piersonii]
GTGAACAAGACCTATATGCTGGACACCTGCATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCCGTTCGTAAGCGCCT
CGAGCAGGCAGTGCTGCGCGGAAACCGCATTGTAATCTCGGCCATCACCTATCAGGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCACCGCGCCACGTGCAGTTGGTCGATGAATTCTGCACCCGCCTTGATGCCGTCCTGCCCTGGGATCGTGCC
GCGGTGGATGCCACCACGGACATCCGAGTCGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATTGC
CGGGCACGCCATCTCGGCTGACGCCGTGCTGGTGACGAACAATACCCGGGAATTTGAGCGGGTTCCGGGTCTGGCGCTGG
AAGACTGGGTTAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT211217 WP_001261286.1 NZ_CP080173:147218-147448 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT211217 NZ_CP104761:5755-5985 [Kalamiella piersonii]
ATGCGCACAGTTTCTATTTTTAAAAACGGCAATAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGAGACAGCATTATCCTGCGCCCCGTCCGGCCGACCTGGAACTCATTTTTTGAACTTG
AAAAAGCCGATCCTGATTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References