Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 142355..142998 Replicon plasmid pPK8566-169kb
Accession NZ_CP080173
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KZY09_RS24335 Protein ID WP_001034046.1
Coordinates 142582..142998 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KZY09_RS24330 Protein ID WP_001261278.1
Coordinates 142355..142585 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS24300 (137865) 137865..138170 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KZY09_RS24305 (138172) 138172..138390 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KZY09_RS24310 (138982) 138982..139470 + 489 WP_011254646.1 hypothetical protein -
KZY09_RS24315 (139504) 139504..140637 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
KZY09_RS24320 (140804) 140804..141577 - 774 WP_000905949.1 hypothetical protein -
KZY09_RS24325 (141590) 141590..142090 - 501 WP_000528932.1 HEPN family nuclease -
KZY09_RS24330 (142355) 142355..142585 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY09_RS24335 (142582) 142582..142998 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY09_RS24340 (143043) 143043..146837 - 3795 WP_001144731.1 hypothetical protein -
KZY09_RS24345 (147218) 147218..147448 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KZY09_RS24350 (147445) 147445..147861 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / tet(A) / sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..169561 169561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T211216 WP_001034046.1 NZ_CP080173:142582-142998 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T211216 NZ_CP104759:c153311-152004 [Kalamiella piersonii]
ATGGATCAGCTCAACGCCTGGATGAATGGCGAACGCGTCGGCACGCTGACGCGCCAGAAAAATGGCGCACATATCTTTCA
GTATGAGCCAGGCTGGCTGAGCAATCCGCTGGCGAGGCCGCTGTCGCTCTCGCTACCGCTGCAGTACCCCGCTATCGCCT
CCGCCAGCGTCATCAACTTCTTTGATAACCTGCTGCCTGATAATCCTCGCGTCAGAGAGCGCATTCTTAACCGTTATCGG
GCGAAGTCGATGCAGCCGTTCGATCTGCTGCGGGAAGTAGGCCGCGACAGCGTCGGCGCGGTAATGCTGCTGCCCGTTGA
GGCCGCCGCGCCCGATCTCTCTGTACGTTATGAACCGCTCGACGCCGCGCGGCTGGAAAAAATACTTTCGGCGTACCAGA
GCGATATTCCGCTGGGGATGATCCCGGAAGAGCGCGACTTCCGCATTTCCGTCGCGGGCGCGCAGGAGAAAACCGCGCTG
CTGAAAACCCCTCAGGGCTGGGCCGTCCCTCAGGGGCCGACGCCCACGTCGCATATCATTAAGCTGCCGATCGGCGAAAT
TAAGCAGCCTTTCGCGACGCTCGATATGCGCGACAGCGTGGAAAACGAATATGTCTGCCTGGCGCTGGCGCGCGAGCTGG
GTTTTCGCGTGCCTGACGCCGCCATTATCACGGCCGGCAAGCTGCGCGCGCTGGCGGTAGAGCGATTCGACCGGCGCTGG
AGCGCTGACGGTGCGCGGCTTTTGCGCCTGCCGCAGGAGGATATTTGCCAGAGCCTCGGCCTCGCCTCCGCGACAAAATA
TGAATCTGACGGCGGGCCGGGCATTGCGGCTATCATGTCGCTGCTGATGGGCTCCAGCAATGCGCTGGAGGATCGCGACG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGCTGATCGGCGCGACCGACGGGCATGCGAAGAACTTTTCGCTCTATATCGAG
CGTGGTGGAAGCTACCGGTTGACGCCGTTCTACGACATTCTTTCCGCTTATCCGGTACTGGGCGGCACCGGCCTCAATAT
TCGCGAACTGAAGCTGGCGATGGGACTCACCGCGTCGAAGGGCAAGAAAGTGGAAATCGATAAAATCTTTCCGCGCCATT
TCCTTGCCACGGCGGCAAGCGTCCGCTTCTCCACCGAACGGATGACGCAGATCCTCGCTTTCTTCGCGGAGGCGTTTCCG
GCCGCCGTGGCGCGCGTCAGGGAGCAACTGCCCGCCGATATCCCGCCCGCAATTGCCGAGGCTATTTTCAGTAACGCGCT
GAAAATGGCGGAGCGGCTTAGCCGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT211216 WP_001261278.1 NZ_CP080173:142355-142585 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT211216 NZ_CP104759:c153547-153311 [Kalamiella piersonii]
ATGATCTACAGCCCACAACAGCTGGCCCACCTGATCAGGCTTGAACGGCAGAAGCGCGGCTGGACGCAGAGCGACCTGGC
GCGCCGGGTCGGCATCAAACAGGCCACCGTGTCGAACTTTGAAAATAATCCTGACAAGACTACGCTAACCACGCTGTTTA
AGATCCTGCAGTCGCTGGAGCAGGAGCTGGTGATTCAGAGTAAATCGACGCAGAGCGGCAAGGAGCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References