Detailed information of TA system TA211215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 137865..138390
• Replicon: plasmid pPK8566-169kb
• Accession: NZ_CP080173
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KZY09_RS24300
• Protein ID: WP_001159868.1
• Coordinates: 137865..138170 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KZY09_RS24305
• Protein ID: WP_000813634.1
• Coordinates: 138172..138390 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS24285 (133797)	133797..134963	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
KZY09_RS24290 (135551)	135551..136306	-	756	WP_000852146.1	replication initiation protein RepE	-
KZY09_RS24295 (137058)	137058..137864	-	807	WP_000016982.1	site-specific integrase	-
KZY09_RS24300 (137865)	137865..138170	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KZY09_RS24305 (138172)	138172..138390	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KZY09_RS24310 (138982)	138982..139470	+	489	WP_011254646.1	hypothetical protein	-
KZY09_RS24315 (139504)	139504..140637	-	1134	WP_000545984.1	DUF3800 domain-containing protein	-
KZY09_RS24320 (140804)	140804..141577	-	774	WP_000905949.1	hypothetical protein	-
KZY09_RS24325 (141590)	141590..142090	-	501	WP_000528932.1	HEPN family nuclease	-
KZY09_RS24330 (142355)	142355..142585	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
KZY09_RS24335 (142582)	142582..142998	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..169561	169561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T211215 WP_001159868.1 NZ_CP080173:c138170-137865 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T211215 NZ_CP104758:2836461-2836679 [Kalamiella piersonii]
ATGAACGATAAGACTCTGACTAAAACTGATTATTTGATGCGGCTGAGACGTTGCCGCTCGATCGATACGCTTGAACGCGT
GATTGAAAAGAATAAGTATGAATTATCCGATGATGAACTGGCGGTATTCTACTCTGCCGCCGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTTTACGACAAAGTCCCTGGCTCAGTATGGAAGTTCGTGCGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT211215 WP_000813634.1 NZ_CP080173:c138390-138172 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT211215 NZ_CP104758:2836060-2836437 [Kalamiella piersonii]
ATGGACGAGTACTCACCGAAACGGCATGATATTGCCCAGCTTAAATTCCTGTGTGAGAACCTGTTTGATGAAAGTATGGC
GACGCTAACCGACAGCCATCACGGCTGGGTTAACGACCCGACTTCAGAGAACAATTTACAGCTTAATGATTTGATTGAGC
ACATTGCTTCTTTCACGATGAATTACAAAATAAAGCATGCTGAAGATGAAGATCTGATAACGCAAATCGACGACTATCTT
GACGATACCTTTATGTTATTCACTAATTACGGTATCAATGCGCAAGATCTGAATCGGTGGCAGCGTTCCGCCCGGCGCTT
ATTTAATCTTTTTAGCGAAGAGTGCGCTTACCTTCAGCAACCCAGCCACTCCATTTAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2