Detailed information of TA system TA211211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 89031..89656
• Replicon: plasmid pPK8566-169kb
• Accession: NZ_CP080173
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZY09_RS23995
• Protein ID: WP_000911313.1
• Coordinates: 89258..89656 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KZY09_RS23990
• Protein ID: WP_000450520.1
• Coordinates: 89031..89258 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS23990 (89031)	89031..89258	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZY09_RS23995 (89258)	89258..89656	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZY09_RS24000 (89665)	89665..91818	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KZY09_RS24005 (92071)	92071..92802	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
KZY09_RS24010 (92834)	92834..93331	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..169561	169561
	-	flank	IS/Tn	-	-	93402..93905	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T211211 WP_000911313.1 NZ_CP080173:89258-89656 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T211211 NZ_CP104757:c1877275-1876991 [Pectobacterium aroidearum]
ATGGAAATTAAATGGCTGCGTAAAGCTGCGGCCAATTTGGAAGCCGAGTACCGCTATATTGCGCAGGACGATCCACAAGC
CGCCAGCCAATTCGTAGATGAAGTCCAAAGGTTGACGGAATTACTACCGCAACAGCCAGCAATGGGAAGACCTGGCCGTG
TACCTGGAACGCGTGAACTGGTGTTAACGCACTATCCCTATATCATCCCCTACCGTGTGAAAGGTAATATGCTCCAGATT
CTGCGCATCTTTCATACACACAGACGTTTACCCGCAAAATGGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT211211 WP_000450520.1 NZ_CP080173:89031-89258 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT211211 NZ_CP104757:c1876944-1876705 [Pectobacterium aroidearum]
ATGGGTAATGTTACTTGCCGTATCGATGACGATCTTAAAAGGCGCTCTTATGCAGCACTGAAGAAACTGGGTATTACGCC
TTCTGAGGCATTGCGCCTTACGTTGGAATACGTTGCTGAAAATGAAAGACTCCCATTTAAACAAAAGTTACTCAATGATG
AAGACGTAGAATTAGTTGAAGTTGTCCGTGAACGTCTTCGCAACCCCAAACCAGTACGCATGACGCTGGATGATCTTTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2