Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4319809..4320395 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KZY09_RS21045 Protein ID WP_042014162.1
Coordinates 4320165..4320395 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KZY09_RS21040 Protein ID WP_000593555.1
Coordinates 4319809..4320168 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS21015 (4315941) 4315941..4316885 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
KZY09_RS21020 (4316882) 4316882..4317715 + 834 WP_105077963.1 nickel ABC transporter permease subunit NikC -
KZY09_RS21025 (4317715) 4317715..4318479 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KZY09_RS21030 (4318476) 4318476..4319282 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KZY09_RS21035 (4319288) 4319288..4319689 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KZY09_RS21040 (4319809) 4319809..4320168 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZY09_RS21045 (4320165) 4320165..4320395 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KZY09_RS21050 (4320499) 4320499..4321623 - 1125 WP_001314210.1 ABC transporter permease -
KZY09_RS21055 (4321623) 4321623..4324358 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T211207 WP_042014162.1 NZ_CP080172:c4320395-4320165 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T211207 NZ_CP104757:c500070-499819 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT211207 WP_000593555.1 NZ_CP080172:c4320168-4319809 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT211207 NZ_CP104757:c499822-499568 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References