Detailed information of TA system TA211204

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3805083..3805918
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8S7N3L3
• Locus tag: KZY09_RS18445
• Protein ID: WP_000854731.1
• Coordinates: 3805541..3805918 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KZY09_RS18440
• Protein ID: WP_016231431.1
• Coordinates: 3805083..3805451 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS18405 (3800725)	3800725..3801609	+	885	WP_219795603.1	50S ribosome-binding GTPase	-
KZY09_RS18410 (3801728)	3801728..3802405	+	678	WP_001097364.1	hypothetical protein	-
KZY09_RS18415 (3802411)	3802411..3802644	+	234	WP_001278281.1	DUF905 family protein	-
KZY09_RS18420 (3802743)	3802743..3803561	+	819	WP_219795604.1	DUF932 domain-containing protein	-
KZY09_RS18425 (3803653)	3803653..3804138	+	486	WP_016231429.1	antirestriction protein	-
KZY09_RS18430 (3804154)	3804154..3804630	+	477	WP_016231430.1	RadC family protein	-
KZY09_RS18435 (3804699)	3804699..3804920	+	222	WP_000692346.1	DUF987 domain-containing protein	-
KZY09_RS18440 (3805083)	3805083..3805451	+	369	WP_016231431.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZY09_RS18445 (3805541)	3805541..3805918	+	378	WP_000854731.1	TA system toxin CbtA family protein	Toxin
KZY09_RS18450 (3805915)	3805915..3806404	+	490	Protein_3628	DUF5983 family protein	-
KZY09_RS18455 (3806416)	3806416..3806613	+	198	WP_000839256.1	DUF957 domain-containing protein	-
KZY09_RS18460 (3806698)	3806698..3807540	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
KZY09_RS18465 (3807821)	3807821..3808357	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
KZY09_RS18470 (3808359)	3808359..3809537	-	1179	WP_000094989.1	type II secretion system protein GspL	-
KZY09_RS18475 (3809534)	3809534..3810511	-	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14230.21 Da
• Isoelectric Point: 7.3526

>T211204 WP_000854731.1 NZ_CP080172:3805541-3805918 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYDLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T211204 NZ_CP104757:c235614-235435 [Pectobacterium aroidearum]
ATGGATAGCAGGACGTTAATCGCAGAAATCAAGGCCGATGGGTGGGAGCTGATACGAGTGAACGGAAGCCACCACCATTT
TATGCACCCCAGCAAACCGGGATTAGTCACAATCCCCCACCCCAAGAAGGATTTACCAATAGGTACGGTAAAAAGCATCA
GGAAACAGGCAGGAATATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13700.50 Da
• Isoelectric Point: 6.7393

>AT211204 WP_016231431.1 NZ_CP080172:3805083-3805451 [Escherichia coli]
VSDTLPGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT211204 NZ_CP104757:c235407-234997 [Pectobacterium aroidearum]
ATGTTTTATCCCATTGCAATTGAAGCAGGCGACGATACTCACGCATACGGCGTAACAGTACCAGATTTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCTATCGCAAACGCTAAAGAAGCGATTACCGGACATATCGAGCTACTGGTTGAGA
TGGGTCAGGATATTCCCACCGTATCAACAGTAGGTCAGCTGGCAAAAGGGTCTGAGTATGCAGGGTACACGTGGGCGGTT
GTTGATATTGATGTAACTCGTTTAATGGGCGGCTCAGAAAAAATTAACGTTACATTGCCCAAATCGCTGATTGACCGCAT
TGATCGTTGCGTGGCGAGTAATCCAGAATTTAAAAGTCGTTCGGGTTTTCTTGCACAGGCGGCATTAGAGCGAATTTCGT
CTTCCCGATGA