Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3589452..3590106 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KZY09_RS17450 Protein ID WP_000244772.1
Coordinates 3589452..3589859 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZY09_RS17455 Protein ID WP_000354046.1
Coordinates 3589840..3590106 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS17430 (3585409) 3585409..3587142 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
KZY09_RS17435 (3587148) 3587148..3587858 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZY09_RS17440 (3587883) 3587883..3588779 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KZY09_RS17445 (3588891) 3588891..3589412 + 522 WP_001055874.1 flavodoxin FldB -
KZY09_RS17450 (3589452) 3589452..3589859 - 408 WP_000244772.1 protein YgfX Toxin
KZY09_RS17455 (3589840) 3589840..3590106 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZY09_RS17460 (3590349) 3590349..3591329 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KZY09_RS17465 (3591406) 3591406..3592065 - 660 WP_000250274.1 hemolysin III family protein -
KZY09_RS17470 (3592229) 3592229..3592540 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
KZY09_RS17475 (3592585) 3592585..3594018 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KZY09_RS17480 (3594075) 3594075..3594818 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T211202 WP_000244772.1 NZ_CP080172:c3589859-3589452 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211202 NZ_CP104756:37479-37790 [Shewanella putrefaciens]
GTGCACGTTATTTCAAGAAAACCATTTAATGATGCCGCCCAAAAGTATCCTAACGATAAGGACGCTTTGGTGCAAATATA
CAGCACCTTACGCAGTGGCACATTCACCAATCCTGATGATTTACGACAAGTATTTCCATCTTTGGATAACTTTAAATATC
GAGATAAATGGTGGGTGATTGATGTGGGGGGCAATAATCTACGAATTATCGCTTTCATCGAGTTCCGAGATAACAGGATG
TATGTAAAGCACGTGGTTAGCCATGCAGACTATAGCAAGCTGACTGACAAGTACCGTCGAACAAAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211202 WP_000354046.1 NZ_CP080172:c3590106-3589840 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211202 NZ_CP104756:37796-38212 [Shewanella putrefaciens]
ATGCTACCTACTGCATGTATTGAAATTAGAGAAGCATTGGCCAAAGTGCCATACCTCGCTCACATTGAAACTCAGGACGA
CTACGAGCAGGCATTGGTATTAATGGACGACCTGGTTGATGACTATGACTCAAACAAATTTTTAATTGAGATGTTGTCAC
TTAGCATTGAGCGATGGGAAGAACAGGCTGATGAATTTGCTGAATTTAATGCTGCCATTGCCGAAATGGACAGTGGTATA
GCTGTATTGAAAACGCTAATGTCTCAGTATCGTCTTGGTGTTGCAGATCTTCCCGAATTAGGCTCAAAAAGTAATGTGAG
TAAGTTACTCAATGCCGCAGAAGGAAAGAAGCTTAATAGACACCACATAGAAGCACTCAGTCAGCGCTTTGGTGTGCCTG
TATCATTATTTTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References