Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3452505..3453088 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KZY09_RS16815 Protein ID WP_000254745.1
Coordinates 3452505..3452840 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KZY09_RS16820 Protein ID WP_000581937.1
Coordinates 3452840..3453088 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS16795 (3447974) 3447974..3448336 + 363 WP_000034929.1 hypothetical protein -
KZY09_RS16800 (3448392) 3448392..3449690 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KZY09_RS16805 (3449778) 3449778..3451415 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KZY09_RS16810 (3451643) 3451643..3452434 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KZY09_RS16815 (3452505) 3452505..3452840 - 336 WP_000254745.1 endoribonuclease MazF Toxin
KZY09_RS16820 (3452840) 3452840..3453088 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KZY09_RS16825 (3453166) 3453166..3455400 - 2235 WP_000226815.1 GTP pyrophosphokinase -
KZY09_RS16830 (3455448) 3455448..3456749 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3436023..3453088 17065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T211201 WP_000254745.1 NZ_CP080172:c3452840-3452505 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T211201 NZ_CP104755:4433013-4433255 [Shewanella putrefaciens]
GTGCAAATATACAGCACCTTACGCAGTGGCACATTCACCAATCCTGATGATTTACGACAAGTATTTCCATCTTTGGATAA
CTTTAAATATCGAGATAAATGGTGGGTGATTGATGTGGGGGGCAATAATCTACGAATTATCGCTTTCATCGAGTTCCGAG
ATAACAGGATGTATGTAAAGCACGTGGTTAGCCATGCAGACTATAGCAAGCTGACTGACAAGTACCGTCGAACAAAGGAG
TAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT211201 WP_000581937.1 NZ_CP080172:c3453088-3452840 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT211201 NZ_CP104755:4433261-4433677 [Shewanella putrefaciens]
ATGCTACCTACTGCATGTATTGAAATTAGAGAAGCATTGGCCAAAGTGCCATACCTCGCTCACATTGAAACTCAGGACGA
CTACGAGCAGGCATTGGTATTAATGGACGACCTGGTTGATGACTATGACTCAAACAAATTTTTAATTGAGATGTTGTCAC
TTAGCATTGAGCGATGGGAAGAACAGGCTGATGAATTTGCTGAATTTAATGCTGCCATTGCCGAAATGGACAGTGGTATA
GCTGTATTGAAAACGCTAATGTCTCAGTATCGTCTTGGTGTTGCAGATCTTCCCGAATTAGGCTCAAAAAGTAATGTGAG
TAAGTTACTCAATGCCGCAGAAGGAAAGAAGCTTAATAGACACCACATAGAAGCACTCAGTCAGCGCTTTGGTGTGCCTG
TATCATTATTTTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References