Detailed information of TA system TA211200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3143690..3144315
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZY09_RS15305
• Protein ID: WP_000911330.1
• Coordinates: 3143690..3144088 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KZY09_RS15310
• Protein ID: WP_000450524.1
• Coordinates: 3144088..3144315 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS15285 (3139568)	3139568..3139768	+	201	WP_000383836.1	YpfN family protein	-
KZY09_RS15290 (3139878)	3139878..3140576	-	699	WP_219795584.1	esterase	-
KZY09_RS15295 (3140650)	3140650..3142665	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
KZY09_RS15300 (3142680)	3142680..3143543	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
KZY09_RS15305 (3143690)	3143690..3144088	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZY09_RS15310 (3144088)	3144088..3144315	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZY09_RS15315 (3144469)	3144469..3145182	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KZY09_RS15320 (3145395)	3145395..3146429	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KZY09_RS15325 (3146446)	3146446..3147324	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KZY09_RS15330 (3147470)	3147470..3148042	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KZY09_RS15335 (3148042)	3148042..3148512	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3081696..3214824	133128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T211200 WP_000911330.1 NZ_CP080172:c3144088-3143690 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T211200 NZ_CP104755:c4333701-4333171 [Shewanella putrefaciens]
GTGAGTTATTCCAAAATTTTCAAAGAATTGGATAAATCACTACACGATAGAGTGTCATTTGACTGTGGCGAAGCAGAGTT
AAACGATTTTATCCAAACTCAAGCAGCAAAACATATGCAAGCAGGTATTAGCCGTACAATGGTTTTACCTGCTGCTATGC
CATTGCCCAATCAAAAATATCCAATTTGCTCATTTTATACTATCGCACCAAGTTCAATTTGCCGTGATACCTTGCCGCAA
GCTATTGGCAAAAAGTTACCACGCTATCCCATTCCGGTCTTTCTGCTTGCTCAACTTGCGGTTCATAAAGAGTTTCATGG
CAGTGGGTTAGGTAAAGCTAGCTTAATCAAAGCACTAGAATATCTGTGGGAAATTAACGCTCACATGAGAGCTTATGCCA
TTGTTGTCGATTGCTTAACTGACCAAGCTGAATCATTCTACGCAAAATATGGTTTCGAGGTTCTTTGTGAAATCAACGGT
CGTGTCAGAATGTTTATTCCAATGAAAACAGTCGGTCAGTTATTCACTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT211200 WP_000450524.1 NZ_CP080172:c3144315-3144088 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT211200 NZ_CP104755:c4333967-4333698 [Shewanella putrefaciens]
ATGGCGACTGCAAGACTTGATATCCGTTTGGATGAAGAAATCAAAGCTAAGGCTGAGAAAGCATCCGCTTTGCTCGGGTT
AAAAAGCTTAACTGAATACGTTGTTCGCTTAATGGACGAAGATGCAACTCAGGTGATAGCTGAGCATGAAAGCGTTACGG
TTAAAGCAGATGTATTCGACCAATTCATAATGGCTTGTGATGAGGCTAAGGTTCCGAATAAGGCATTGCGTGAAGCGGCT
GCATTTACTAAAAATGGTGAGTTTAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33