Detailed information of TA system TA211190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2086335..2086973
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KZY09_RS10270
• Protein ID: WP_000813794.1
• Coordinates: 2086797..2086973 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZY09_RS10265
• Protein ID: WP_001270286.1
• Coordinates: 2086335..2086751 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS10245 (2081487)	2081487..2082428	-	942	WP_001251319.1	ABC transporter permease	-
KZY09_RS10250 (2082429)	2082429..2083442	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KZY09_RS10255 (2083460)	2083460..2084605	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KZY09_RS10260 (2084850)	2084850..2086256	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
KZY09_RS10265 (2086335)	2086335..2086751	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZY09_RS10270 (2086797)	2086797..2086973	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZY09_RS10275 (2087195)	2087195..2087425	+	231	WP_000494244.1	YncJ family protein	-
KZY09_RS10280 (2087517)	2087517..2089478	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZY09_RS10285 (2089551)	2089551..2090087	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
KZY09_RS10290 (2090179)	2090179..2091354	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2091394..2092659	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T211190 WP_000813794.1 NZ_CP080172:c2086973-2086797 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211190 NZ_CP104746:19827-20234 [Erwinia pyrifoliae]
ATGGATGCACTGAGTGTCATGGGAATTTATTTAACATCTGAATTTGAGACAGAACGCCAGAAAGCCCGCATCAGCAATGC
CATGTTGCGCAAAGCTGCTAAGGCTATTTTTTCTGGTCTGCCTGGTGATCCACTTGGAAAATTCACCTTTAAAAAGCGGC
TCGGCTTGCCCGGAGTAGGAGCCAGAGATGGAGCACGGTCAATCGTTTTTTTCAATGATGGTGACAACATCTTCTTCTTT
GATATGTACCTGAAAAGTGGACTTTCAAAAAAGAAAGGAAAGGAGCTTGAAGATGATGAAATTGATGCTTACTGCAATAT
CGCAAAAGATTTTATCTCAATGAGTCCGGCAACTATAAATAAGCTACTGTCTGATAAAGAACTGGTCGAGGTAAATTGTG
ATGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT211190 WP_001270286.1 NZ_CP080172:c2086751-2086335 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211190 NZ_CP104746:20227-20544 [Erwinia pyrifoliae]
ATGACTGAACGTCTGAAACGCATTCAAGGTATGGCTGAACGCTATAACAAACTAGGCGTGGTAAAAGATGAAACCGTAGC
TGCAATTGATGCAGCGGTCGCAGCCCGTAATATCCCGGCGATCCGGGTCATGACCGGCAGCCAGATAAAGGAAGTCCGCA
CCCGATACAACATGTCGCAAGCCGCATTGGCTATAACAACCGGCATGTCCGTAGAAAGCGTCTCCAAATGGGAGCGAAAC
GAAAGCAAACCAAATAAGGCTGCTCTGCGGATCATTAACATCATCGATACTAAAGGGCCACAGGTGTTCATGGCATAA

Structures

Toxin

• PDB: 6HPB