Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1027524..1028142 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KZY09_RS04960 Protein ID WP_001291435.1
Coordinates 1027524..1027742 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KZY09_RS04965 Protein ID WP_000344800.1
Coordinates 1027768..1028142 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS04925 (1022813) 1022813..1023385 + 573 WP_000779831.1 YbaY family lipoprotein -
KZY09_RS04930 (1023416) 1023416..1023727 - 312 WP_000409911.1 MGMT family protein -
KZY09_RS04940 (1024106) 1024106..1024459 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KZY09_RS04945 (1024501) 1024501..1026051 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KZY09_RS04950 (1026215) 1026215..1026685 - 471 WP_000136192.1 YlaC family protein -
KZY09_RS04955 (1026801) 1026801..1027352 - 552 WP_000102568.1 maltose O-acetyltransferase -
KZY09_RS04960 (1027524) 1027524..1027742 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KZY09_RS04965 (1027768) 1027768..1028142 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KZY09_RS04970 (1028688) 1028688..1031837 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KZY09_RS04975 (1031860) 1031860..1033053 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T211182 WP_001291435.1 NZ_CP080172:c1027742-1027524 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T211182 NZ_CP104733:4667142-4667429 [Pectobacterium sp. F1-1]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGTGCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTGCGGCAAAGTTACGAGGAATGAAGGACTGCTACAAAATAA
AGCTCCGGGCGTCAGGCTTTCGTTTGGTTTACGAAGTCATTGACGACATATTGGTTATCGCCGTGGTGGCAGTAGGCAAA
CGTGAGCGCAGCGGCGTCTATAATCTGGCAAGTGAGCGGATGAGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT211182 WP_000344800.1 NZ_CP080172:c1028142-1027768 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT211182 NZ_CP104733:4666894-4667145 [Pectobacterium sp. F1-1]
ATGCCAAATATTATATTGAGTGATACCAGTGCTAGTGTCAGTGAACTGAAAAAGAACCCAATGGCGACGGTCAATGCTGG
CGATGGCCTGCCTGTCGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAGGAGTTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References