Detailed information of TA system TA211181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 993228..994065
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KZY09_RS04790
• Protein ID: WP_000227784.1
• Coordinates: 993228..993770 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KZY09_RS04795
• Protein ID: WP_001297137.1
• Coordinates: 993754..994065 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS04770 (989069)	989069..989560	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
KZY09_RS04775 (989688)	989688..991052	-	1365	WP_001000978.1	MFS transporter	-
KZY09_RS25790 (991400)	991400..991537	+	138	Protein_936	sel1 repeat family protein	-
KZY09_RS04780 (991593)	991593..992290	+	698	WP_223400810.1	IS1-like element IS1X2 family transposase	-
KZY09_RS04785 (992315)	992315..993172	+	858	WP_001609455.1	tetratricopeptide repeat protein	-
KZY09_RS04790 (993228)	993228..993770	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KZY09_RS04795 (993754)	993754..994065	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KZY09_RS04800 (994250)	994250..995140	-	891	WP_000971336.1	heme o synthase	-
KZY09_RS04805 (995152)	995152..995481	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KZY09_RS04810 (995481)	995481..996095	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KZY09_RS04815 (996085)	996085..998076	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KZY09_RS04820 (998098)	998098..999045	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	991787..992290	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T211181 WP_000227784.1 NZ_CP080172:c993770-993228 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T211181 NZ_CP104733:c4369537-4369052 [Pectobacterium sp. F1-1]
ATGGTTGATAAGCGAGTAATAAATGGGTGGCAGCTATTTACGCATCCTTGTTTTGATGTGCAGTTTGAGTCTCTTATTGT
TGAAGTTGAACAATTGAGAAAAAAACACCCAGAATCTTATCAGAAAAAGCAGGCAACTAAGCTATTGGCTGCAATAGTCA
AAGTCATCCGTACTGAGATTTGTGGCGATCCGTCCCAGACGAAGTTTAGACAAGGCGATACGTTAGGCGAAGGCAACAAA
CATTGGTTCAGGGCAAAATTTCTGGGTCAGTATCGGTTATTTTTTCGCTATAGCGAGAAACATAAAACTATCATCCTTGC
CTGGGTCAATGATTCAGACACTAAACGAGCATATGGCGATCAGCATGATGCTTATAAAGTTTTTAGCGGCATGCTAGCAA
GCGGGAATCCTCCGGGAGATTGGAAGCAATTGCTTGCTGAATCCTATAAGGAGACCATGCTTACGCAAATGCTCGTGCGG
CCATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT211181 WP_001297137.1 NZ_CP080172:c994065-993754 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT211181 NZ_CP104733:c4369887-4369543 [Pectobacterium sp. F1-1]
ATGTTTAATTTAAAATCAGCGGATGTATTGTTGCGCGCCGATAGCCGCTTAACGGCGCGTAGTCAGACAACGATTCCATC
GTCTGTGCGTGATGCCATGAACCTGCAACCTGGGGAGTACATTCATTATAGCGTGCTACCGGGTGGGCAGGTGCTGATCA
GTCGGCGTGAAGAGGCTGTAGATGACGATCCCGTTATTCATCGTTTTCTGCATTTTTTGGCTGAAGATATGCAACATCAT
CCAGAAAATATTAAGCCGTTTGATCAATCGCTTATGTCACGTGCCAGTGTGTTAACCGCAGGGATGGATATTGATCTGGA
TGCACCGCTTACTGACGACGAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3