Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 157936..158579 Replicon plasmid pPK8275-180kb
Accession NZ_CP080165
Organism Escherichia coli strain PK8275

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KZY08_RS26515 Protein ID WP_001034044.1
Coordinates 158163..158579 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KZY08_RS26510 Protein ID WP_001261286.1
Coordinates 157936..158166 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY08_RS26495 (153073) 153073..153303 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KZY08_RS26500 (153300) 153300..153716 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KZY08_RS26505 (153761) 153761..157555 - 3795 WP_001144731.1 hypothetical protein -
KZY08_RS26510 (157936) 157936..158166 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY08_RS26515 (158163) 158163..158579 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY08_RS26520 (158654) 158654..160219 + 1566 WP_001128474.1 AAA family ATPase -
KZY08_RS26525 (160204) 160204..161226 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / dfrA14 / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..180290 180290
- flank IS/Tn - - 161480..161983 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T211173 WP_001034044.1 NZ_CP080165:158163-158579 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T211173 NZ_CP104733:c513996-513742 [Pectobacterium sp. F1-1]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTTTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT211173 WP_001261286.1 NZ_CP080165:157936-158166 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT211173 NZ_CP104733:c514244-513993 [Pectobacterium sp. F1-1]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCTAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTTTGGAAGAGTATAACTCTCTAGAAGAAACCGCGTATT
TACTGCGTTCACCAGCCAATGCCAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References