Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 153073..153716 Replicon plasmid pPK8275-180kb
Accession NZ_CP080165
Organism Escherichia coli strain PK8275

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KZY08_RS26500 Protein ID WP_001034046.1
Coordinates 153300..153716 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KZY08_RS26495 Protein ID WP_001261278.1
Coordinates 153073..153303 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY08_RS26465 (148583) 148583..148888 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KZY08_RS26470 (148890) 148890..149108 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KZY08_RS26475 (149700) 149700..150188 + 489 WP_011254646.1 hypothetical protein -
KZY08_RS26480 (150222) 150222..151355 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
KZY08_RS26485 (151522) 151522..152295 - 774 WP_000905949.1 hypothetical protein -
KZY08_RS26490 (152308) 152308..152808 - 501 WP_000528932.1 HEPN family nuclease -
KZY08_RS26495 (153073) 153073..153303 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY08_RS26500 (153300) 153300..153716 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY08_RS26505 (153761) 153761..157555 - 3795 WP_001144731.1 hypothetical protein -
KZY08_RS26510 (157936) 157936..158166 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KZY08_RS26515 (158163) 158163..158579 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / dfrA14 / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..180290 180290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T211172 WP_001034046.1 NZ_CP080165:153300-153716 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T211172 NZ_CP104733:473321-474655 [Pectobacterium sp. F1-1]
ATGCCTCGAACACAACAGCGTTTAGCAATATGGATGAATGGAATTCAAGTGGGATTCTGGGAAAAGACCCGAGGCGAAGA
TAAGTTGCAATATCTTCCCGAATGGATCGCTGATGAACAGGGAAGACCTTTATCGCTTTCTTTGCTTTTCACCCCCGGTA
ATCAGCTTTGGCGCGGCAATGTGGTACGCGACTATTTTGATAATTTATTGCCTGACAGCGAAGGTATACGCAGGCGTTTA
GCAACGCGTTACCACGCTGATAGCCTTGAGCCTTTTGATCTGTTGGCTGAGCTGGGAAGAGACTGTGTTGGCGCAATACA
GTTACTGAATGGTAATGAGGAGCCCACAGATCTATTTTCCGTAAATTATCGCCCACTTTGTGAAGCTGAGATCGCCACTA
TATTGCGTAATACAACAGCGGCATTGCTGCCAGGTCGGCAGGATGATACTGACGATTTACGTTTATCGATTGCCGGTGCG
CAGGAAAAAACGGCTTTACTCTGGCATGAAAGGCAATGGTGTTTGCCAGAAGGGAGTACCCCTACGACGCATATTTTCAA
GTTACCACTCGGGCTAGTGGGTAATATGCAAGCGGATATGAGTACGTCGGTTGAAAATGAATGGCTGTGTTCTTTGATCC
TTAAGGAATATGGGATTCCTGTCGCAAAAACACAGATTGCACAGTTTGAAGAGCAGAAGGCACTGGTTGTTGAGCGTTTT
GATAGAAGATGGTCAAGCGATCAGCAATGGATCATTCGCTTGCCACAAGAGGATATGTGTCAGGCTCTGGGTGTTTCCCC
GCTACGAAAATACCAGTCTGATGGTGGGCCGGGGATTTCCGATATTATGACCATACTGAGTCATTCGGAACAGGCTGAGC
AGGACAAAGCGCAGTTTTTCAGGGCGCAAATTATTTTCTGGCTGATAGCGGCTACTGATGGGCACGCCAAAAACTTCAGT
ATCGCTATTGAGCCACAAGGTCGCTATCACCTTACGCCGCTTTATGATGTTTTATCGGCATGGCCGGTCATTGGTCCGCG
TAATAACCAGATTTCCTGGCAGAAGTGCAAGCTGGCAATGGCTGTTCGGGGTAGTAGTAATTATTACCATCTGTACAGAA
TTCAACGGCGGCATTGGGTCAATCATGGTGAATTAACTGGTCTGGGTAGACGACAGGTTGAAGCCATGATGGATGATATT
ATATCTAGGACACCTGAGGTTATTGAACGTGTATCTTCGTTGCTGCCTGAGTCATTCCCACCAGAGCTTGCGGAATGTGT
TTTTGACGGTATGCGACAACAGTGTGGTCGGTTACAAGAATTGCACCGTAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT211172 WP_001261278.1 NZ_CP080165:153073-153303 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT211172 NZ_CP104733:473011-473316 [Pectobacterium sp. F1-1]
ATGAATACAGTCTATCCGTTGAAAACCTTGAATCAGTTGCGCCCATTGCTGGTTGGCTTTCGCAAAGCTAAGGGGCTGAC
GCAAAAAGACGTGTCTGAAAGGTTAGGTGTAACACAACAAACCTATGCCCGTTTGGAAGCAAACCCCGGAAGTGCCAGTA
TTGAGCGCTTGTTTAAAGTGCTCACCGTTCTGGGGGTTGAGGTGGTGCTGTCTTCTGGACCAAGAGTACATTTCTCTATG
TCATCCTCAACAATGGAAGAGCCAGAAAAGTCAATAGGCTCACCCGCCAGACGGGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References