Detailed information of TA system TA211167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 99213..99838
• Replicon: plasmid pPK8275-180kb
• Accession: NZ_CP080165
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZY08_RS26155
• Protein ID: WP_000911313.1
• Coordinates: 99440..99838 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KZY08_RS26150
• Protein ID: WP_000450520.1
• Coordinates: 99213..99440 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS26150 (99213)	99213..99440	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KZY08_RS26155 (99440)	99440..99838	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZY08_RS26160 (99847)	99847..102000	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KZY08_RS26165 (102253)	102253..102984	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
KZY08_RS26170 (103016)	103016..103513	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..180290	180290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T211167 WP_000911313.1 NZ_CP080165:99440-99838 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T211167 NZ_CP104727:7352589-7352870 [Pseudomonas citronellolis]
ATGAGCCTGCAGTGGACGCACAAGGCGGCGGCCGACCTGGACGGCCTGTACGACCACTACGTGGTGCTGATCGGCCCGGA
GAAGGCGCTCAAGGCCATCCAGGACGTGGTCGGCCAGGTGAAGGCGCTGGCCGACCTCAGCCTGAGCAGCGTCGGCCGGC
CCAGCGAGGTGCCCGGCGTGCGCGAATTGCCGCTGGAACGCTGGCCCTACCAGGCCGCCTACCGGGTAAAAGGCCGCGAT
GTACAAATTTTGCGCATCGACTCTGTGGATAACCCGGGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT211167 WP_000450520.1 NZ_CP080165:99213-99440 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT211167 NZ_CP104727:7352365-7352592 [Pseudomonas citronellolis]
TTGACCACCGTAATCTCCTTCCGCGCCGATGACGCCCTGGCCAAGGCCCTGGACCAGCTGGCCCGGGCCACCCACCGGGA
CCGCCCCTACCACCTGCGCCAGGCCCTGGCGCAGTACCTGGAGCGCCAGAGCTGGCACGTGGCGGCCATCGACGAGGGCC
TGGCCGACGCCAACGCCGGGCACCTGCTGCCGCACGCGGAGATAGAAGCCAAGTGGGGGCTGGCATGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2