Detailed information of TA system TA211157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3628196..3628980
• Replicon: chromosome
• Accession: NZ_CP080163
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: KZY08_RS17510
• Protein ID: WP_000613626.1
• Coordinates: 3628486..3628980 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: KZY08_RS17505
• Protein ID: WP_001110447.1
• Coordinates: 3628196..3628489 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS17495 (3623346)	3623346..3624305	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
KZY08_RS17500 (3624878)	3624878..3628063	+	3186	WP_000827427.1	ribonuclease E	-
KZY08_RS17505 (3628196)	3628196..3628489	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
KZY08_RS17510 (3628486)	3628486..3628980	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
KZY08_RS17515 (3629075)	3629075..3630028	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
KZY08_RS17520 (3630040)	3630040..3631683	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
KZY08_RS17525 (3631749)	3631749..3632690	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
KZY08_RS17530 (3632690)	3632690..3633787	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T211157 WP_000613626.1 NZ_CP080163:3628486-3628980 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T211157 NZ_CP104727:c1449086-1448832 [Pseudomonas citronellolis]
ATGAATAGCCGGCAGCGGGCGACCCTGGAAGCGCTCTACGCGGTCCCTACGCCATGCAACCTGGAATGGGCACGCATTGA
ATCCCTGCTGCTCTCGCTGGGCGCCAGGCTTATCGAAGGCAACGGCTCGCGCGTGCGCTTCGAGCTGAATGGCGTCATCG
CCAGCTTCCACCGCCCGCACCCGGCCAAGGAAGCCAAACCCTATCAGGTCCGCGATGCGCGGGCCTTCATCGAACGAGCG
GGATACGCACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT211157 WP_001110447.1 NZ_CP080163:3628196-3628489 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT211157 NZ_CP104727:c1448835-1448509 [Pseudomonas citronellolis]
ATGAGCCCGATGAAATACAAAGGCTATGTCGCACGCATCGAGTACAGCGACGACGACGGGCTGTTCATCGGCCATATCGC
CGGCATCCAGGACGTCATCGGTTTCCATGGCGAGTCGGTGAGCGAACTGCGCCAGTTGTTCCATGAGGCCGTCGACGACT
ACCTCGACACCTGCGCCAAGCTCGGCCGCGAGCCACAGAAGCCCTACTCCGGCAAGCTCAGCCTGCGCCTGGACCCGGCA
CTGCATGCCCGCGTCGCGCTCAAGGCGGAGCTGAGCAACAAGAGCATCAACCAGTGGGTGGCGGAGCGGCTGAGCGAAGC
GGTGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6