Detailed information of TA system TA211156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3282832..3283470
• Replicon: chromosome
• Accession: NZ_CP080163
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: KZY08_RS15710
• Protein ID: WP_000813797.1
• Coordinates: 3283294..3283470 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZY08_RS15705
• Protein ID: WP_076611057.1
• Coordinates: 3282832..3283248 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS15685 (3277984)	3277984..3278925	-	942	WP_001251335.1	ABC transporter permease	-
KZY08_RS15690 (3278926)	3278926..3279939	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
KZY08_RS15695 (3279957)	3279957..3281102	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
KZY08_RS15700 (3281347)	3281347..3282753	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
KZY08_RS15705 (3282832)	3282832..3283248	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZY08_RS15710 (3283294)	3283294..3283470	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZY08_RS15715 (3283692)	3283692..3283922	+	231	WP_000494244.1	YncJ family protein	-
KZY08_RS15720 (3284014)	3284014..3285975	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZY08_RS15725 (3286048)	3286048..3286584	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
KZY08_RS15730 (3286676)	3286676..3287848	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T211156 WP_000813797.1 NZ_CP080163:c3283470-3283294 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211156 NZ_CP104727:1432088-1432384 [Pseudomonas citronellolis]
ATGGAGAAATCGACACCCCATTGCCGTCTCGATCGAGTCAAGTCGCTGCTTGCCCAGGGCAGGGTTCGCGCGACCTATTC
CGCGCTTTCCGGCGGCGCGGCGCTGGGGTTGGACTTCGCCGACATGTTGGCGGTCATCGAGGCCCTGGCGCCTCGCGATT
TCCACAAGAGCATGACGACTCACGCCGATCATCGGGTCTGGCAGGACGTCTACCGACCTTCGACCGCCGTGGGGCGGATC
TATCTGAAGTTGACCGTCATAGACGATGTGCTTGTCGTGTCCTTCAAGGAGCTATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT211156 WP_076611057.1 NZ_CP080163:c3283248-3282832 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211156 NZ_CP104727:1432387-1432788 [Pseudomonas citronellolis]
ATGAAATGTCCCCTCTGCGGTGGAGCCGAACTGCTCCACGAGACCCGCGACCTTCCCTATGCCTACAAGGGGGAGAGCAC
CCTGATAACGGGTGTGCGCGGTGAGTTCTGCCCGGCCTGTGGCGAGGCCGTGCTGGACGCCGACGAGTCGGCGCGGGTGA
GTGCCCAGATGCTGGCCTTCAACAAGCGGGTGAACGCTTCGATGGTCGATCCGGGATTCATTGCCGCGGTACGCAAGAAG
CTGGCGCTGGACCAGCGCGAGGCCGCGGAGATCTTCGGTGGCGGCGTGAATGCGTTCTCCCGCTACGAGAATGGCAAGAC
GCGCCCGCCGTTGGCGCTGGTCAAGCTGCTCAAGGTGCTCGACCGCCATCCCGAATTGCTGGAAGAGGTGCGCGCGGGGT
AA

Structures

Toxin

• AlphaFold DB: A0A829J715