Detailed information of TA system TA211154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3183097..3184685
• Replicon: chromosome
• Accession: NZ_CP080163
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KZY08_RS15265
• Protein ID: WP_001546825.1
• Coordinates: 3183363..3184685 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KZY08_RS15260
• Protein ID: WP_001296726.1
• Coordinates: 3183097..3183363 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS15260 (3183097)	3183097..3183363	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KZY08_RS15265 (3183363)	3183363..3184685	+	1323	WP_001546825.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KZY08_RS15270 (3185083)	3185083..3185982	-	900	WP_001057776.1	LysR family transcriptional regulator	-
KZY08_RS15275 (3186122)	3186122..3186430	+	309	WP_001221046.1	hypothetical protein	-
KZY08_RS15280 (3186439)	3186439..3187128	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
KZY08_RS15285 (3187202)	3187202..3188149	+	948	WP_000854796.1	aldo/keto reductase	-
KZY08_RS15290 (3188252)	3188252..3188407	+	156	WP_001322415.1	hypothetical protein	-
KZY08_RS15295 (3188367)	3188367..3188786	+	420	WP_001322416.1	amidohydrolase family protein	-
KZY08_RS28595 (3188776)	3188776..3188928	+	153	WP_001322417.1	amidohydrolase family protein	-
KZY08_RS15305 (3189079)	3189079..3189651	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.00 Da
• Isoelectric Point: 8.1369

>T211154 WP_001546825.1 NZ_CP080163:3183363-3184685 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHCRLSREYGIK

Nucleotide

• Length: 1323 bp

>T211154 NZ_CP104727:977032-977397 [Pseudomonas citronellolis]
ATGGTGAAGGCGCTGTTCGACACCAACATCCTCATCGACTACCTCAACGGCCACGAACAGGCCCGCGACGAGCTGCGCCG
CTACGATGACCCGGCCATCAGCATCGTCACCTGGATGGAGGTGATGATCGGCGCCACGCCCGCCACCGCCGCGGCCACCC
GCGCCTTCCTCGACAGCTTCGCCCTGGTGCCGCTCGACGCGTCCGTCGCCGAGCGCGCCGTGGCGGTGCGCCAGGCGCTG
CGGGTGAAGCTGCCGGACGCCATCATCAAGGCCAGCGCCGAGGTGCAGGGGCGCCTGCTGGTCACCCGCAACACCAGGGA
CTTCCCCGTCGACGACGCCGGCGTGCGCCTGCCCTACCGGCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT211154 WP_001296726.1 NZ_CP080163:3183097-3183363 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT211154 NZ_CP104727:976823-977038 [Pseudomonas citronellolis]
ATGCGTACCCTGGTCGACATCCCTGCCGAACACCTCGAAGCGCTCAATGCCCTGGGCGAGCAACAGCAGCAATCGCGCGC
CGCGCTGATCCGCGAGGCGGTCGCCGAGTACCTGGTCCGGCACCGCCGGCAGGATGCCGAGGCCGCCTTCGGCCTGTGGA
ACGGCAGCGCCGGCGACGGCCTGGCCTACCAGGAGAAGGTGCGCGAGGAATGGTGA