Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 137900..138397 Replicon chromosome
Accession NZ_CP080163
Organism Escherichia coli strain PK8275

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZY08_RS00730 Protein ID WP_001173343.1
Coordinates 137900..138073 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZY08_RS00735 Protein ID WP_000398619.1
Coordinates 138101..138397 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY08_RS00720 (134571) 134571..136028 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
KZY08_RS00725 (136265) 136265..137782 + 1518 WP_000003806.1 lysine--tRNA ligase -
KZY08_RS00730 (137900) 137900..138073 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZY08_RS00735 (138101) 138101..138397 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZY08_RS00740 (138626) 138626..138898 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KZY08_RS00745 (138910) 138910..139140 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
KZY08_RS00750 (139321) 139321..140952 + 1632 WP_001216477.1 sensor histidine kinase -
KZY08_RS00755 (140949) 140949..141668 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 108729..138397 29668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T211138 WP_001173343.1 NZ_CP080163:c138073-137900 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T211138 NZ_CP104721:2717893-2718000 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT211138 WP_000398619.1 NZ_CP080163:c138397-138101 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT211138 NZ_CP104721:c2717846-2717780 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References