Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58167..58692 Replicon plasmid pPK8261-145kb
Accession NZ_CP080158
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KZY07_RS26935 Protein ID WP_001159871.1
Coordinates 58387..58692 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KZY07_RS26930 Protein ID WP_000813630.1
Coordinates 58167..58385 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS26910 (KZY07_26915) 53501..54443 + 943 Protein_58 hexose kinase -
KZY07_RS26915 (KZY07_26920) 54496..55485 + 990 WP_072643615.1 tagatose-bisphosphate aldolase -
KZY07_RS26920 (KZY07_26925) 55800..57028 + 1229 WP_085959875.1 IS3 family transposase -
KZY07_RS26925 (KZY07_26930) 57054..57185 - 132 Protein_61 transposase -
KZY07_RS26930 (KZY07_26935) 58167..58385 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KZY07_RS26935 (KZY07_26940) 58387..58692 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KZY07_RS26940 (KZY07_26945) 58693..59502 + 810 WP_045145707.1 site-specific integrase -
KZY07_RS26945 (KZY07_26950) 59675..60028 + 354 WP_000864812.1 colicin M immunity protein -
KZY07_RS26950 (KZY07_26955) 60078..60894 - 817 Protein_66 lipid II-degrading bacteriocin colicin M -
KZY07_RS26955 (KZY07_26960) 61136..61663 + 528 WP_032353548.1 colicin B immunity protein -
KZY07_RS26960 (KZY07_26965) 61681..62343 - 663 Protein_68 colicin-like pore-forming protein -
KZY07_RS26965 (KZY07_26970) 62401..63098 + 698 WP_103215986.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..145740 145740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T211135 WP_001159871.1 NZ_CP080158:58387-58692 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T211135 NZ_CP104721:2110769-2110872 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT211135 WP_000813630.1 NZ_CP080158:58167-58385 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT211135 NZ_CP104721:c2110910-2110639 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References