Detailed information of TA system TA211134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 20886..21445
• Replicon: plasmid pPK8261-145kb
• Accession: NZ_CP080158
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KZY07_RS26735
• Protein ID: WP_219795304.1
• Coordinates: 20886..21161 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0H2XL20
• Locus tag: KZY07_RS26740
• Protein ID: WP_012006537.1
• Coordinates: 21161..21445 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS26715 (KZY07_26725)	17601..18327	-	727	Protein_18	IS256 family transposase	-
KZY07_RS26720 (KZY07_26730)	18435..18815	+	381	WP_001171523.1	transposase	-
KZY07_RS26725 (KZY07_26735)	18812..19159	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
KZY07_RS26730 (KZY07_26740)	19209..20747	+	1539	WP_219795284.1	IS66-like element ISEc8 family transposase	-
KZY07_RS26735 (KZY07_26745)	20886..21161	-	276	WP_219795304.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZY07_RS26740 (KZY07_26750)	21161..21445	-	285	WP_012006537.1	ribbon-helix-helix domain-containing protein	Antitoxin
KZY07_RS26745 (KZY07_26755)	21946..22536	+	591	WP_000086536.1	recombinase family protein	-
KZY07_RS26750 (KZY07_26760)	22628..23632	+	1005	WP_000427619.1	IS110-like element IS5075 family transposase	-
KZY07_RS26755 (KZY07_26765)	23910..24068	+	159	Protein_26	integrase core domain-containing protein	-
KZY07_RS26760 (KZY07_26770)	24218..25216	+	999	WP_032353474.1	ABC transporter substrate-binding protein	-
KZY07_RS26765 (KZY07_26775)	25216..26253	+	1038	WP_032353476.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..145740	145740
	-	inside	IScluster/Tn	-	-	5986..23632	17646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.49 Da
• Isoelectric Point: 9.9324

>T211134 WP_219795304.1 NZ_CP080158:c21161-20886 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKLAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T211134 NZ_CP104721:1902693-1902947 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10501.96 Da
• Isoelectric Point: 6.4632

>AT211134 WP_012006537.1 NZ_CP080158:c21445-21161 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT211134 NZ_CP104721:1902445-1902696 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H2XL20