Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 1820..2355 Replicon plasmid pPK8261-145kb
Accession NZ_CP080158
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name relE Uniprot ID A0A0D8WEY8
Locus tag KZY07_RS26640 Protein ID WP_032353555.1
Coordinates 2068..2355 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WHE0
Locus tag KZY07_RS26635 Protein ID WP_045145550.1
Coordinates 1820..2071 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS26625 (KZY07_26635) 1..873 + 873 WP_045145554.1 incFII family plasmid replication initiator RepA -
KZY07_RS26630 (KZY07_26640) 1566..1733 + 168 WP_045145553.1 hypothetical protein -
KZY07_RS26635 (KZY07_26645) 1820..2071 + 252 WP_045145550.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZY07_RS26640 (KZY07_26650) 2068..2355 + 288 WP_032353555.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZY07_RS26645 (KZY07_26655) 2365..2652 + 288 WP_045145547.1 hypothetical protein -
KZY07_RS26650 (KZY07_26660) 3771..4259 + 489 WP_072157196.1 serine acetyltransferase -
KZY07_RS26655 (KZY07_26665) 4539..5132 - 594 WP_032353553.1 ProQ/FINO family protein -
KZY07_RS26660 (KZY07_26670) 5986..7174 - 1189 Protein_7 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..145740 145740
- inside IScluster/Tn - - 5986..23632 17646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11114.16 Da        Isoelectric Point: 10.6261

>T211133 WP_032353555.1 NZ_CP080158:2068-2355 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T211133 NZ_CP104721:1902445-1902696 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9219.48 Da        Isoelectric Point: 4.1789

>AT211133 WP_045145550.1 NZ_CP080158:1820-2071 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHYVDLD
SYL

Download         Length: 252 bp

>AT211133 NZ_CP104721:1902693-1902947 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WEY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WHE0

References