Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 153101..153744 Replicon plasmid pPK8261-180kb
Accession NZ_CP080157
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KZY07_RS26510 Protein ID WP_001034046.1
Coordinates 153328..153744 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KZY07_RS26505 Protein ID WP_001261278.1
Coordinates 153101..153331 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS26475 (148611) 148611..148916 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KZY07_RS26480 (148918) 148918..149136 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KZY07_RS26485 (149728) 149728..150216 + 489 WP_011254646.1 hypothetical protein -
KZY07_RS26490 (150250) 150250..151383 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
KZY07_RS26495 (151550) 151550..152323 - 774 WP_000905949.1 hypothetical protein -
KZY07_RS26500 (152336) 152336..152836 - 501 WP_000528932.1 HEPN family nuclease -
KZY07_RS26505 (153101) 153101..153331 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY07_RS26510 (153328) 153328..153744 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY07_RS26515 (153789) 153789..157583 - 3795 WP_001144731.1 hypothetical protein -
KZY07_RS26520 (157964) 157964..158194 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KZY07_RS26525 (158191) 158191..158607 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / dfrA14 / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..180318 180318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T211131 WP_001034046.1 NZ_CP080157:153328-153744 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T211131 NZ_CP104721:929590-929997 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT211131 WP_001261278.1 NZ_CP080157:153101-153331 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT211131 NZ_CP104721:929343-929609 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References