Detailed information of TA system TA211114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3266758..3267463
• Replicon: chromosome
• Accession: NZ_CP080155
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: KZY07_RS15815
• Protein ID: WP_000539521.1
• Coordinates: 3266758..3267144 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KZY07_RS15820
• Protein ID: WP_001280945.1
• Coordinates: 3267134..3267463 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS15795 (3262762)	3262762..3263388	+	627	WP_001295292.1	glutathione S-transferase GstB	-
KZY07_RS15800 (3263385)	3263385..3264500	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
KZY07_RS15805 (3264611)	3264611..3264994	-	384	WP_000497137.1	biofilm formation regulator BssR	-
KZY07_RS15810 (3265207)	3265207..3266532	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KZY07_RS15815 (3266758)	3266758..3267144	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZY07_RS15820 (3267134)	3267134..3267463	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KZY07_RS15825 (3267533)	3267533..3268861	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
KZY07_RS15830 (3268869)	3268869..3271217	-	2349	WP_001322378.1	EAL domain-containing protein	-
KZY07_RS15835 (3271395)	3271395..3272306	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T211114 WP_000539521.1 NZ_CP080155:3266758-3267144 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T211114 NZ_CP104692:137895-138182 [Enterobacter hormaechei]
ATGGCATATTTTCTGGATTTTGACGAGCGTGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGCTAAA
AAATAAGTTGGCTGAAGTGCTTGAGTCACCCCGAATTGATGCGAACAAGCTTCGGGGTATGCATGACTGTTACAAAATTA
AGCTCCGGTCTTCAGGCTATCGTCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAGCGCTCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT211114 WP_001280945.1 NZ_CP080155:3267134-3267463 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT211114 NZ_CP104692:137656-137895 [Enterobacter hormaechei]
ATGGGTAGCATCAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCTGCGCTTGAAAAAATGGGTGTAACTCC
CTCTGAGGCACTTCGCCTCATGCTTGAATATATTGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AGGATGCTGAGCTCGTGGAGATCGTGAAAGAACGACTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6