Detailed information of TA system TA211113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3019553..3020337
• Replicon: chromosome
• Accession: NZ_CP080155
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: KZY07_RS14645
• Protein ID: WP_000613626.1
• Coordinates: 3019843..3020337 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: KZY07_RS14640
• Protein ID: WP_001110447.1
• Coordinates: 3019553..3019846 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS14630 (3014703)	3014703..3015662	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
KZY07_RS14635 (3016235)	3016235..3019420	+	3186	WP_000827427.1	ribonuclease E	-
KZY07_RS14640 (3019553)	3019553..3019846	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
KZY07_RS14645 (3019843)	3019843..3020337	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
KZY07_RS14650 (3020432)	3020432..3021385	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
KZY07_RS14655 (3021397)	3021397..3023040	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
KZY07_RS14660 (3023106)	3023106..3024047	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
KZY07_RS14665 (3024047)	3024047..3025144	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T211113 WP_000613626.1 NZ_CP080155:3019843-3020337 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T211113 NZ_CP104692:c66562-66077 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT211113 WP_001110447.1 NZ_CP080155:3019553-3019846 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT211113 NZ_CP104692:c66816-66550 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6