Detailed information of TA system TA211112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2674189..2674827
• Replicon: chromosome
• Accession: NZ_CP080155
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: KZY07_RS12845
• Protein ID: WP_000813797.1
• Coordinates: 2674651..2674827 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KZY07_RS12840
• Protein ID: WP_076611057.1
• Coordinates: 2674189..2674605 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS12820 (2669341)	2669341..2670282	-	942	WP_001251335.1	ABC transporter permease	-
KZY07_RS12825 (2670283)	2670283..2671296	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
KZY07_RS12830 (2671314)	2671314..2672459	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
KZY07_RS12835 (2672704)	2672704..2674110	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
KZY07_RS12840 (2674189)	2674189..2674605	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KZY07_RS12845 (2674651)	2674651..2674827	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KZY07_RS12850 (2675049)	2675049..2675279	+	231	WP_000494244.1	YncJ family protein	-
KZY07_RS12855 (2675371)	2675371..2677332	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KZY07_RS12860 (2677405)	2677405..2677941	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
KZY07_RS12865 (2678033)	2678033..2679205	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T211112 WP_000813797.1 NZ_CP080155:c2674827-2674651 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T211112 NZ_CP104691:4558206-4558691 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAACGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT211112 WP_076611057.1 NZ_CP080155:c2674605-2674189 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT211112 NZ_CP104691:4557952-4558218 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A829J715