Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 999614..1000268 Replicon chromosome
Accession NZ_CP080155
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag KZY07_RS04930 Protein ID WP_000244769.1
Coordinates 999861..1000268 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KZY07_RS04925 Protein ID WP_000354046.1
Coordinates 999614..999880 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS04905 (995702) 995702..997135 - 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -
KZY07_RS04910 (997180) 997180..997491 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
KZY07_RS04915 (997655) 997655..998314 + 660 WP_000250269.1 hemolysin III family protein -
KZY07_RS04920 (998391) 998391..999371 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
KZY07_RS04925 (999614) 999614..999880 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KZY07_RS04930 (999861) 999861..1000268 + 408 WP_000244769.1 protein YgfX Toxin
KZY07_RS04935 (1000308) 1000308..1000829 - 522 WP_001055874.1 flavodoxin FldB -
KZY07_RS04940 (1000941) 1000941..1001837 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
KZY07_RS04945 (1001862) 1001862..1002572 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KZY07_RS04950 (1002578) 1002578..1004311 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T211100 WP_000244769.1 NZ_CP080155:999861-1000268 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T211100 NZ_CP104689:31723-32037 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT211100 WP_000354046.1 NZ_CP080155:999614-999880 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT211100 NZ_CP104689:32037-32453 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGTGCG
CCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCTGCCAGATTTGGGGTATCACCTG
CGTTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References