Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 148760..149372 Replicon chromosome
Accession NZ_CP080155
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag KZY07_RS00635 Protein ID WP_000833473.1
Coordinates 148760..148945 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID L4IWR9
Locus tag KZY07_RS00640 Protein ID WP_000499744.1
Coordinates 148962..149372 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS00625 (145592) 145592..146743 + 1152 WP_000741501.1 L-threonine dehydrogenase -
KZY07_RS00630 (146944) 146944..148482 + 1539 WP_001309845.1 aldehyde dehydrogenase AldB -
KZY07_RS00635 (148760) 148760..148945 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
KZY07_RS00640 (148962) 148962..149372 + 411 WP_000499744.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZY07_RS00645 (149444) 149444..151408 - 1965 WP_001026861.1 glycoside hydrolase family 127 protein -
KZY07_RS00650 (151419) 151419..152819 - 1401 WP_000204802.1 MFS transporter -
KZY07_RS00655 (153045) 153045..153860 + 816 WP_000891825.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T211095 WP_000833473.1 NZ_CP080155:148760-148945 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T211095 NZ_CP104681:3884870-3885247 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15241.15 Da        Isoelectric Point: 4.5486

>AT211095 WP_000499744.1 NZ_CP080155:148962-149372 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT211095 NZ_CP104681:3884460-3884819 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB L4IWR9

References