Detailed information of TA system TA211095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 148760..149372
• Replicon: chromosome
• Accession: NZ_CP080155
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: KZY07_RS00635
• Protein ID: WP_000833473.1
• Coordinates: 148760..148945 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: KZY07_RS00640
• Protein ID: WP_000499744.1
• Coordinates: 148962..149372 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS00625 (145592)	145592..146743	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
KZY07_RS00630 (146944)	146944..148482	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
KZY07_RS00635 (148760)	148760..148945	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
KZY07_RS00640 (148962)	148962..149372	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KZY07_RS00645 (149444)	149444..151408	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
KZY07_RS00650 (151419)	151419..152819	-	1401	WP_000204802.1	MFS transporter	-
KZY07_RS00655 (153045)	153045..153860	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T211095 WP_000833473.1 NZ_CP080155:148760-148945 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T211095 NZ_CP104681:3884870-3885247 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT211095 WP_000499744.1 NZ_CP080155:148962-149372 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT211095 NZ_CP104681:3884460-3884819 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9