Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21117..21760 Replicon plasmid pPK8276-100kb
Accession NZ_CP080151
Organism Escherichia coli strain PK8276

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KZY06_RS25580 Protein ID WP_001044768.1
Coordinates 21344..21760 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KZY06_RS25575 Protein ID WP_001261287.1
Coordinates 21117..21347 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY06_RS25555 (16277) 16277..17365 + 1089 WP_000952217.1 transcriptional repressor PifC -
KZY06_RS25560 (17367) 17367..19592 + 2226 WP_000698737.1 P-loop NTPase fold protein -
KZY06_RS25565 (19642) 19642..20541 - 900 WP_000963206.1 nucleotide-binding protein -
KZY06_RS25570 (20531) 20531..20821 - 291 WP_000111771.1 hypothetical protein -
KZY06_RS25575 (21117) 21117..21347 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KZY06_RS25580 (21344) 21344..21760 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KZY06_RS25585 (21922) 21922..24060 - 2139 WP_000350638.1 AAA family ATPase -
KZY06_RS25590 (24414) 24414..24671 + 258 WP_000343085.1 hypothetical protein -
KZY06_RS25595 (24671) 24671..25261 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / aph(3')-Ia / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / sitABCD - 1..100987 100987


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T211091 WP_001044768.1 NZ_CP080151:21344-21760 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T211091 NZ_CP104681:3589016-3589234 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT211091 WP_001261287.1 NZ_CP080151:21117-21347 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT211091 NZ_CP104681:3588616-3588990 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References