Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 35957..36755 Replicon plasmid pPK8276-110kb
Accession NZ_CP080149
Organism Escherichia coli strain PK8276

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag KZY06_RS24430 Protein ID WP_000072677.1
Coordinates 36234..36755 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag KZY06_RS24425 Protein ID WP_001351987.1
Coordinates 35957..36226 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY06_RS24400 (KZY06_24385) 31493..32833 - 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -
KZY06_RS24405 (KZY06_24390) 32877..33617 - 741 WP_001717320.1 hypothetical protein -
KZY06_RS24410 (KZY06_24395) 33811..34566 - 756 WP_021533207.1 DUF2971 domain-containing protein -
KZY06_RS24415 (KZY06_24400) 34611..34964 - 354 WP_160378290.1 hypothetical protein -
KZY06_RS24420 (KZY06_24405) 34970..35638 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
KZY06_RS24425 (KZY06_24410) 35957..36226 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
KZY06_RS24430 (KZY06_24415) 36234..36755 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
KZY06_RS24435 (KZY06_24420) 36924..37175 - 252 WP_000901559.1 hypothetical protein -
KZY06_RS24440 (KZY06_24425) 37177..37869 - 693 WP_012640731.1 hypothetical protein -
KZY06_RS24445 (KZY06_24430) 37883..38206 - 324 WP_000064175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110377 110377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T211085 WP_000072677.1 NZ_CP080149:36234-36755 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T211085 NZ_CP104681:2354871-2355158 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT211085 WP_001351987.1 NZ_CP080149:35957-36226 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT211085 NZ_CP104681:2354632-2354871 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References