Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3152569..3153145 Replicon chromosome
Accession NZ_CP080146
Organism Escherichia coli strain PK8276

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KZY06_RS15165 Protein ID WP_001297643.1
Coordinates 3152858..3153145 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KZY06_RS15160 Protein ID WP_000063160.1
Coordinates 3152569..3152871 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY06_RS15145 (3149165) 3149165..3151315 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
KZY06_RS15150 (3151365) 3151365..3151568 + 204 WP_000467859.1 YbdD/YjiX family protein -
KZY06_RS15155 (3151579) 3151579..3152535 + 957 WP_001297640.1 GTPase -
KZY06_RS15160 (3152569) 3152569..3152871 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KZY06_RS15165 (3152858) 3152858..3153145 - 288 WP_001297643.1 BrnT family toxin Toxin
KZY06_RS15170 (3153344) 3153344..3154040 - 697 Protein_2983 IS1 family transposase -
KZY06_RS15175 (3154355) 3154355..3155587 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
KZY06_RS15180 (3155628) 3155628..3156908 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3153344..3153553 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T211075 WP_001297643.1 NZ_CP080146:c3153145-3152858 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T211075 NZ_CP104679:c125915-125490 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT211075 WP_000063160.1 NZ_CP080146:c3152871-3152569 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT211075 NZ_CP104679:c126142-125912 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References