Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2643771..2644608 Replicon chromosome
Accession NZ_CP080146
Organism Escherichia coli strain PK8276

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag KZY06_RS12725 Protein ID WP_000227786.1
Coordinates 2644066..2644608 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag KZY06_RS12720 Protein ID WP_001297137.1
Coordinates 2643771..2644082 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY06_RS12695 (2638791) 2638791..2639738 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
KZY06_RS12700 (2639760) 2639760..2641751 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KZY06_RS12705 (2641741) 2641741..2642355 + 615 WP_206356481.1 cytochrome o ubiquinol oxidase subunit III -
KZY06_RS12710 (2642355) 2642355..2642684 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KZY06_RS12715 (2642696) 2642696..2643586 + 891 WP_000971336.1 heme o synthase -
KZY06_RS12720 (2643771) 2643771..2644082 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
KZY06_RS12725 (2644066) 2644066..2644608 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
KZY06_RS12730 (2644664) 2644664..2645599 - 936 WP_001297127.1 tetratricopeptide repeat protein -
KZY06_RS12735 (2645712) 2645712..2646408 + 697 WP_001390273.1 IS1-like element IS1A family transposase -
KZY06_RS12740 (2646791) 2646791..2648155 + 1365 WP_001000978.1 MFS transporter -
KZY06_RS12745 (2648283) 2648283..2648774 - 492 WP_001138904.1 nucleotide binding protein YajQ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T211072 WP_000227786.1 NZ_CP080146:2644066-2644608 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T211072 NZ_CP104678:c5343001-5342618 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT211072 WP_001297137.1 NZ_CP080146:2643771-2644082 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT211072 NZ_CP104678:c5343243-5343001 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References