Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 28532..29176 Replicon plasmid pPK8241-33kb
Accession NZ_CP080143
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag KZY05_RS29130 Protein ID WP_000833471.1
Coordinates 28994..29176 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X7JR29
Locus tag KZY05_RS29125 Protein ID WP_052989980.1
Coordinates 28532..28969 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS29105 (KZY05_29115) 25943..26278 - 336 WP_000963832.1 DUF6290 family protein -
KZY05_RS29110 (KZY05_29120) 26624..27031 + 408 WP_064261963.1 hypothetical protein -
KZY05_RS29115 (KZY05_29125) 27041..27802 + 762 WP_219788500.1 conjugal transfer protein TraL -
KZY05_RS29120 (KZY05_29130) 27799..28503 + 705 WP_001279864.1 hypothetical protein -
KZY05_RS29125 (KZY05_29135) 28532..28969 - 438 WP_052989980.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KZY05_RS29130 (KZY05_29140) 28994..29176 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
KZY05_RS29135 (KZY05_29145) 29614..29892 - 279 WP_000545934.1 hypothetical protein -
KZY05_RS29140 (KZY05_29150) 29950..30279 - 330 WP_000449469.1 hypothetical protein -
KZY05_RS29145 (KZY05_29155) 30719..30961 - 243 WP_001450803.1 hypothetical protein -
KZY05_RS29150 (KZY05_29160) 30951..31217 - 267 WP_000868275.1 hypothetical protein -
KZY05_RS29155 (KZY05_29165) 31268..31492 - 225 WP_000814384.1 hypothetical protein -
KZY05_RS29160 (KZY05_29170) 31590..31799 - 210 WP_000866037.1 hypothetical protein -
KZY05_RS29165 (KZY05_29175) 31840..32163 - 324 WP_000427402.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33837 33837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T211057 WP_000833471.1 NZ_CP080143:c29176-28994 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T211057 NZ_CP104677:c4097863-4097579 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16199.09 Da        Isoelectric Point: 4.3135

>AT211057 WP_052989980.1 NZ_CP080143:c28969-28532 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVKESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT211057 NZ_CP104677:c4098095-4097853 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X7JR29

References