Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 42907..43442 | Replicon | plasmid pPK8241-82kb |
| Accession | NZ_CP080142 | ||
| Organism | Escherichia coli strain PK8241 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q3YTD6 |
| Locus tag | KZY05_RS28755 | Protein ID | WP_000222766.1 |
| Coordinates | 43155..43442 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | KZY05_RS28750 | Protein ID | WP_001132900.1 |
| Coordinates | 42907..43158 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZY05_RS28715 (37959) | 37959..38702 | + | 744 | WP_039002216.1 | conjugal transfer pilus acetylase TraX | - |
| KZY05_RS28720 (38757) | 38757..39317 | + | 561 | WP_000139310.1 | fertility inhibition protein FinO | - |
| KZY05_RS28725 (39452) | 39452..39664 | + | 213 | WP_001541896.1 | ANR family transcriptional regulator | - |
| - (40006) | 40006..40067 | - | 62 | NuclAT_1 | - | - |
| - (40006) | 40006..40067 | - | 62 | NuclAT_1 | - | - |
| - (40006) | 40006..40067 | - | 62 | NuclAT_1 | - | - |
| - (40006) | 40006..40067 | - | 62 | NuclAT_1 | - | - |
| KZY05_RS28730 (40111) | 40111..40260 | + | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| KZY05_RS28735 (40528) | 40528..40785 | + | 258 | WP_001541895.1 | replication regulatory protein RepA | - |
| KZY05_RS28740 (41017) | 41017..41091 | + | 75 | WP_061357229.1 | RepA leader peptide Tap | - |
| KZY05_RS28745 (41084) | 41084..41941 | + | 858 | WP_039002220.1 | incFII family plasmid replication initiator RepA | - |
| KZY05_RS28750 (42907) | 42907..43158 | + | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| KZY05_RS28755 (43155) | 43155..43442 | + | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KZY05_RS28760 (43517) | 43517..43705 | - | 189 | Protein_55 | IS3 family transposase | - |
| KZY05_RS28765 (43739) | 43739..44239 | - | 501 | Protein_56 | Tn3 family transposase | - |
| KZY05_RS28770 (44273) | 44273..45571 | - | 1299 | Protein_57 | Tn3-like element Tn3 family transposase | - |
| KZY05_RS28775 (45639) | 45639..46343 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| KZY05_RS28780 (46397) | 46397..46891 | - | 495 | Protein_59 | IS4-like element ISVsa5 family transposase | - |
| KZY05_RS28785 (47034) | 47034..47894 | + | 861 | WP_219788497.1 | aminoglycoside N-acetyltransferase AAC(3)-IId | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aac(3)-IId / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) | - | 1..82038 | 82038 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11147.23 Da Isoelectric Point: 10.5832
>T211056 WP_000222766.1 NZ_CP080142:43155-43442 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T211056 NZ_CP104677:3600594-3600869 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGACGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATTTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGACGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATTTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT211056 WP_001132900.1 NZ_CP080142:42907-43158 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT211056 NZ_CP104677:c3600859-3600447 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAAATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCGT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACTGTGCCCCCCTCTCCCCGGCCAGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAAATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCGT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACTGTGCCCCCCTCTCCCCGGCCAGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CDZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0VIP2 |