Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7053..7546 Replicon plasmid pPK8241-MCR1
Accession NZ_CP080141
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag KZY05_RS28175 Protein ID WP_001230709.1
Coordinates 7053..7307 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag KZY05_RS28180 Protein ID WP_000151456.1
Coordinates 7304..7546 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS28135 (KZY05_28150) 2083..2244 + 162 WP_001128311.1 hypothetical protein -
KZY05_RS29640 2570..2722 + 153 Protein_3 DUF5431 family protein -
KZY05_RS28140 (KZY05_28155) 2667..2789 + 123 WP_223685802.1 Hok/Gef family protein -
KZY05_RS28145 (KZY05_28160) 2939..3535 + 597 WP_000125165.1 ProQ/FINO family protein -
KZY05_RS28150 (KZY05_28165) 3553..3837 - 285 WP_001162371.1 hypothetical protein -
KZY05_RS28155 (KZY05_28170) 3911..4576 - 666 WP_000532149.1 division plane positioning ATPase MipZ -
KZY05_RS28160 (KZY05_28175) 5165..5494 + 330 WP_001542022.1 hypothetical protein -
KZY05_RS28165 (KZY05_28180) 5895..6410 + 516 WP_001025393.1 J domain-containing protein -
KZY05_RS28170 (KZY05_28185) 6514..7056 + 543 WP_001083904.1 hypothetical protein -
KZY05_RS28175 (KZY05_28190) 7053..7307 + 255 WP_001230709.1 hypothetical protein Toxin
KZY05_RS28180 (KZY05_28195) 7304..7546 + 243 WP_000151456.1 hypothetical protein Antitoxin
KZY05_RS28185 (KZY05_28200) 8117..8410 + 294 WP_000673428.1 hypothetical protein -
KZY05_RS28190 (KZY05_28205) 8899..9147 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
KZY05_RS28195 (KZY05_28210) 9144..9491 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
KZY05_RS29645 9579..10022 - 444 WP_225930715.1 hypothetical protein -
KZY05_RS28205 (KZY05_28220) 10263..10562 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
KZY05_RS28210 (KZY05_28225) 10822..11148 - 327 WP_000206697.1 hypothetical protein -
KZY05_RS28215 (KZY05_28230) 11150..11473 - 324 WP_223666488.1 hypothetical protein -
KZY05_RS29650 11450..11590 - 141 Protein_20 hypothetical protein -
KZY05_RS28220 (KZY05_28235) 11610..12317 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..62272 62272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-82)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T211048 WP_001230709.1 NZ_CP080141:7053-7307 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T211048 NZ_CP104667:83155-83337 [Escherichia coli]
GTGAAGTATTCAGAGTTCAAACGCTGGTTAATCCAGCAAGGGGCAGAGTTCAGGAAAGCGCCTGGTGGCGGCAGTCATCA
GAAAGTGAACCTGAACGGAAAGCGGTCGGTTTTCCCGGACCACGGTTCAAAGGAAATGCCTGAACCGCTACGCAAGAAAA
TAATGAAGGACCTGGGGCTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT211048 WP_000151456.1 NZ_CP080141:7304-7546 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT211048 NZ_CP104667:83368-83799 [Escherichia coli]
ATGTTTAACTATCCGGTAAAACTGGAGCGCGACGACAAAACCGGCGCGTATGTCGTATCATGCCGCGACCTACCATTGTT
TAATTCCGTGGGTGACTCAGTTGAAGAAGCGTTGCTTGAGGCTGGTTATGGATTGGTCGCAGCGGTTGCGATTGAGATTG
AAGAACGCCGACCTGTTCCCACTGGTAGCGAGCCAAAAGAAGGTGAGTACGTTGTCTCTTTGCCAGTGCTACCAGCAATG
AAGGCTGCACTCCACAACGCGATGATAGAAACCGGCACACGCAAAGCAGAGTTGGCGCGCAAACTCGGAAAAAATGGAAC
ACAGATTGATCGGCTTCTCGACGTCGAACATTCATCGAAAGTTGAAACTGTCGAGCTGGCACTGCATCAACTGAATAAAA
ATATCGCCGTATCTGTCACACCAATGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References